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Process for extracting pleocidin from fermentation liquor of saccharopolyspora spinosa

A technology of Saccharopolyspora spinosa and spinosad, which is applied in the field of spinosad extraction, can solve the problems of difficulty in adapting to large-scale industrial production, high equipment requirements, and long time consumption, and achieve mature equipment development, good product quality, and low labor costs. low intensity effect

Inactive Publication Date: 2010-12-08
HUNAN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Although the resin adsorption extraction method has a good effect to a certain extent, such as high purity, the macroporous resin adsorption extraction process currently has small adsorption capacity, difficult desorption, small processing capacity, high equipment requirements, and many processes. It is difficult to adapt to the requirements of large-scale industrial production due to defects such as long time consumption and high cost
[0007] At present, in the prior art, there is no spinosad extraction process with high yield, good purity, low cost, easy operation and convenient industrial production.

Method used

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  • Process for extracting pleocidin from fermentation liquor of saccharopolyspora spinosa
  • Process for extracting pleocidin from fermentation liquor of saccharopolyspora spinosa
  • Process for extracting pleocidin from fermentation liquor of saccharopolyspora spinosa

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Embodiment 1

[0022] (1) Add 5 mol / L NaOH solution to the fermentation broth in the fermenter to adjust the pH of the fermentation broth to 9.5, then heat the fermentation broth to 60°C, heat and stir for 35 minutes; (2) After cooling the fermentation broth to room temperature Add methanol, the volume ratio of fermentation broth to methanol is 1:1.5, stir and extract at 30°C for 8 hours, discharge the material to a plate and frame filter press, filter and collect the filtrate to obtain the extract clear liquid; (3) extract clear The liquid was transferred to a rotary evaporator for vacuum concentration and volatilization to completely remove methanol to obtain a spinosad concentrated solution with a yield of 98.3%; (4) add butyl acetate to the spinosad concentrated solution for extraction, and the extraction conditions were: two-phase volume ratio ( Extraction solvent / spinosyn concentrated solution) = 1:1, the temperature is room temperature 25°C, pH = 9.0, and the extraction time is 10 min;...

Embodiment 2

[0024](1) Add 3 mol / L NaOH solution to the fermentation broth in the fermenter, adjust the pH of the fermentation broth to 10.5, heat the fermentation broth to 65°C, and heat and stir for 45 minutes; (2) After cooling the fermentation broth to room temperature Add methanol, the volume ratio of fermentation broth to methanol is 1:1, stir and extract at 30°C for 12 hours, then discharge the material to a plate and frame filter press, filter and collect the filtrate to obtain the extract clear liquid; (3) extract The supernatant liquid was transferred to a vacuum concentration tank to volatilize and remove methanol to obtain a spinosad concentrated solution with a yield of 98.4%; (4) Add butyl acetate to extract the spinosad concentrated solution, using a CTL70-N centrifugal extractor with a drum diameter of 70 mm, rotation speed 2500 r / min, extraction conditions: volume ratio of two phases (extraction solvent / spinosyn concentrate) = 1:1, temperature 30°C, pH = 8.5; (5) The CTL70...

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Abstract

The invention discloses a process for extracting pleocidin from fermentation liquor of saccharopolyspora spinosa, which comprises the following steps of: (1) preprocessing fermentation liquor; (2) adding a high dielectric constant polar organic solvent into the fermentation liquor for soaking and extracting the pleocidin, performing solid-liquid separation and collecting clear leach liquor; (3) removing the high dielectric constant polar organic solvent by vacuum concentration and volatilization to obtain the concentrated solution of the pleocidin; (4) adding a low dielectric constant or highalcohol extraction solvent for extraction to obtain a loaded organic phase; (5) adding acid water into the loaded organic phase to perform reextraction, and collecting a reextraction phase; and (6) removing the residual extraction solvent in the reextraction phase by volatilization, adjusting the pH value to 8.5 to 11.5 by using solution of sodium hydroxide (NaOH), allowing the pleocidin to precipitate, filtering, washing the pleocidin precipitate for 1 to 3 times by using diluted alkali liquor, and drying the washed product under vacuum to obtain pleocidin powder. The process for extracting pleocidin from fermentation liquor of saccharopolyspora spinosa has a total yield of over 80 percent and has the advantages of high efficiency, high quality, low cost, easy industrialization and the like.

Description

technical field [0001] The invention relates to a process for extracting antibiotics from fermented liquid, in particular to a process for extracting spinosads from Saccharopolyspora spinosa fermented liquid by extraction-reextraction method. Background technique [0002] Spinosyn (spinosad, spinosyns), also known as spinosyn and spinosad, is an actinomycete Saccharopolyspora spinosa isolated from soil samples. Saccharopolyspora spinosa ) is a secondary metabolite produced by aerobic fermentation, which is a macrolide antibiotic. Its molecular structure contains a 22-membered lactone ring, a rhamnose and a forosamine, and its solubility in water is very small. It is easily soluble in various polar and non-polar organic solvents such as alcohols and esters. After aerobic fermentation, the fermentation broth of Saccharopolyspora spinosa mainly contains two insecticidal active ingredients, spinosyn A (85-90%) and D (10-15%), and also contains a small amount of B, C, and E , F...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/08C07H1/08
Inventor 夏立秋丁学知余子全杨尉伍维
Owner HUNAN NORMAL UNIVERSITY
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