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Reticulocyte mimics and preparation method thereof

A technology of reticulocytes and simulants, which is applied in the field of blood quality control substances, can solve the problems of reduced membrane strength, low cell ratio, complicated operation steps and the like, and achieves the effects of industrialized production, low cost and simple steps.

Active Publication Date: 2010-11-10
SHENZHEN MINDRAY BIO MEDICAL ELECTRONICS CO LTD
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

The problem is that due to the low proportion of cells effectively injected with nucleic acid, the yield of reticulocytes is low, and the prepared product is a mixture of erythrocytes and reticulocytes, and the membrane strength of the cells is reduced due to expansion and shrinkage. , In addition, the operation steps are cumbersome, making this scheme difficult to be used in the production of quality control products

Method used

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  • Reticulocyte mimics and preparation method thereof
  • Reticulocyte mimics and preparation method thereof
  • Reticulocyte mimics and preparation method thereof

Examples

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preparation example Construction

[0027] The preparation method comprises the following steps: washing and separating anticoagulated blood containing anucleated red blood cells to obtain purified anucleated red blood cells;

[0028] adding a cell treatment solution and a fixative, the cell treatment solution contains a protein denaturant and an optional osmotic agent and / or a surfactant, and the surface and / or intracellular substances of the anucleated red blood cells are treated and fixed ;

[0029] The resulting product is washed.

[0030] In one embodiment of the present invention, plasma and platelets in the blood are removed by centrifugation or standing, and white blood cells are removed by a white blood cell filter or other equivalent means to obtain purified anucleated red blood cells.

[0031] The blood used in the embodiment of the present invention can be selected from any human or animal blood containing anucleated cells, as long as the anucleated erythrocytes of animals that are similar to the me...

Embodiment 1

[0085] Take fresh anticoagulated bovine whole blood, and every 800ml of bovine blood contains 200ml of anticoagulant. The anticoagulated whole blood was centrifuged at 2400 rpm for 5 minutes. Remove the supernatant, wash with washing solution, and centrifuge again at 2400 rpm for 5 minutes. And repeat the wash one or two times. Remove the supernatant and filter through a leukocyte strainer to remove leukocytes. Use cell treatment solution A to adjust the red blood cell concentration of the above filtrate to 1×10 12 pc / L, stir and mix for 1-5 minutes, add fixative A to a final concentration of 2% (the volume ratio of the fixative to the total reaction solution, the same below), and act for 5 hours at room temperature, during which the mixture is constantly mixed and stirred. Repeat washing the reaction product three to four times with the washing solution. Suspend the product with an appropriate amount of preservation solution, and then mix it with other cell simulants in p...

Embodiment 2

[0087] Take fresh anticoagulated human whole blood. The anticoagulated whole blood was centrifuged at 2400 rpm for 5 minutes. Remove the supernatant, wash with washing solution, and centrifuge again at 2400 rpm for 5 minutes. And repeat the wash one or two times. Remove the supernatant and filter through a leukocyte strainer to remove leukocytes. Adjust the red blood cell concentration of the above filtrate to 1×10 with cell treatment solution B 12 pc / L, stir and mix for 1-5 minutes, add fixative A to a final concentration of 2%, react at room temperature for 5 hours, and keep mixing and stirring during this period. Repeat washing the reaction product three to four times with the washing solution. Suspend the product with an appropriate amount of preservation solution, and then mix it with other cell simulants in proportion to prepare a hematology analyzer quality control substance as described above for the preparation of the full-component quality control substance. The...

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Abstract

On one aspect, the invention provides a method for preparing reticulocyte mimics, and on the other aspect, the invention provides reticulocyte mimics which is obtained from the method. On another aspect, the invention provides a whole blood quality control substance which contains the reticulocyte mimics provided by the invention.

Description

technical field [0001] The invention relates to a blood quality control substance. More specifically, the present invention relates to a method for preparing a reticulocyte simulant, a reticulocyte simulant obtained by the method, and a whole blood quality control substance containing the reticulocyte simulant. Background technique [0002] A blood control is a liquid containing single or multiple components of blood cells or blood cell simulants, with detectable properties like blood, used to routinely monitor the accuracy and precision of blood analyzers. [0003] Reticulocyte-related parameters are an important piece of information provided by mid- and high-end blood cell analyzers. Reticulocytes are immature red blood cells that have just denucleated and still have a small amount of RNA. The total number or percentage of reticulocytes is very valuable for the clinical diagnosis and curative effect observation of anemia. It is one of the commonly done projects in hemat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/96G01N33/49
CPCG01N33/96G01N33/80
Inventor 王璐徐祖越夏涛
Owner SHENZHEN MINDRAY BIO MEDICAL ELECTRONICS CO LTD
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