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Novel chitosan nanoparticles and preparation method thereof

A technology of nanoparticles and chitosan, applied in food preparation, food science, application and other directions, can solve the problem of chitosan nanoparticles intolerance to strong acid, etc., and achieve the effect of mild and strong stability of encapsulation and protection

Inactive Publication Date: 2010-09-22
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

However, as an oral delivery carrier, chitosan nanoparticles are not resistant to strong acids, and will dissociate and degrade rapidly under low pH conditions, resulting in the destruction of the delivered nutrients in the gastric acid environment and cannot be targeted for absorption in the intestinal tract

Method used

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  • Novel chitosan nanoparticles and preparation method thereof
  • Novel chitosan nanoparticles and preparation method thereof

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Embodiment Construction

[0007] 1. Weigh 50g of casein, add 500mL of 0.05M NaOH solution, stir continuously with a magnetic stirrer, and adjust the pH to 8.0 after it is fully dissolved. Weigh 0.25 g of trypsin and dissolve it in 0.75 mL of deionized water. Add the trypsin solution into the casein-sodium hydroxide solution, mix well, place in a 50°C water bath for constant temperature reaction, and constantly adjust the pH of the hydrolyzate with 1M NaOH solution to keep the pH of the hydrolyzate at 8.0. When the pH of the hydrolyzate was adjusted back to 8.0 for the last time and remained unchanged for more than half an hour, the reaction was considered to be over. After the reaction was over, the hydrolyzate was bathed in boiling water for 5 minutes to kill the enzyme. After the temperature of the hydrolyzate returns to room temperature, adjust the pH of the hydrolyzate to 4.6 with 1M HCl, centrifuge at 2000r / min for 30min, and take the supernatant, which is the CPP crude extraction product. Adjus...

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Abstract

The invention discloses a method capable of being used for preparing novel chitosan-casein phosphopeptide nanoparticles in the food industry. At present, the chitosan nanoparticles are generally prepared by a method using sodium tripolyphosphate cross-linked chitosan. However, as oral delivery carriers, the chitosan nanoparticles are not resistant to strong acid and cause that delivered nutriments are damaged in gastric acid and cannot be delivered to intestinal tracts in a targeted mode for absorption. In the method, a product obtained by using trypsase to hydrolyze casein is separated and purified to obtain casein phosphopeptide, and the casein phosphopeptide and chitosan are cross-linked with each other to from a 'core-shell' nanoparticle structure through condition optimization. Underthe action of negative charges of a surface phosphate group, the chitosan-casein phosphopeptide nanoparticles can avoid acidic and enzymatic degradations in vivo to have very strong stability, have certain targeting and controlled-release characteristics, and solve the problem that the current chitosan-sodium tripolyphosphate nanoparticles serving as the oral delivery carriers can be degraded by the gastric acid easily.

Description

technical field [0001] The patent of the invention relates to a method for preparing novel chitosan-casein phosphopeptide nanoparticles in the food industry. Background technique [0002] Casein Phosphopeptides (CPP) are peptides containing clustered phospho-seryl groups obtained from bovine casein as raw material, hydrolyzed by a single enzyme or a complex enzyme system, and then separated and purified. Can and Ca 2+ binds, prevents calcium phosphate precipitation, and makes the small intestine sensitive to Ca 2+ The solubility is greatly increased. Calcium, iron and zinc play an important role in animals, because the feed raw materials contain a large amount of phytate, cellulose, uronic acid, sodium alginate and oxalic acid, etc. Absorption. CPP can increase the solubility of calcium, iron, zinc and other minerals to promote the absorption of minerals. In addition, CPP also plays an important role in animal immunity and reproduction. [0003] Chitosan has been widel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08L89/00C08L5/08C08J3/24C12P21/06A23L1/30A23L33/10
Inventor 曾晓雄胡冰王舒舒孙怡
Owner NANJING AGRICULTURAL UNIVERSITY
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