Process for preparing oxytetracycline single colony frozen bacteria

Abstract

The invention discloses a process for preparing oxytetracycline single colony frozen bacteria, which comprises the following steps of: preparing a plating medium from 3.5 to 4.5 percent of whole meal flour, 0.1 to 0.3 percent of yeast extract powder, 0.1 to 0.3 percent of monopotassium phosphate, 1.5 to 2.5 percent of agar and the balance of water in a certain proportion; cultivating single colony; preparing single colony frozen bacteria from the cultivated single colony; and performing shaking preliminary screening and shaking secondary screening to select the single colony frozen bacteria with strong production capacity to finally prepare freeze-drying tube strains for production. The plating medium can cultivate the single colony with large colony diameter and large numbers of conidium. Single colony spore suspension is directly used for preparing the single colony frozen bacteria. The process of the invention reduces passage times of the strain in the rejuvenation breeding process and the preparation process, prevents strain degeneration, improves the preservation effect and finally fulfills the aim of improving the working efficiency, increasing the yield and reducing the consumption.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation engineering, and in particular relates to a preparation process of oxytetracycline single colony frozen bacteria. Background technique [0002] Oxytetracycline (Oxytetracycline) is a tetracyclic broad-spectrum antibiotic. Domestic large-scale fermentation production began in the 1960s, and it has become one of the largest antibiotic fermentation products in my country. It has a wide range of applications in medical care, animal husbandry and agriculture. use. The market prospect of oxytetracycline products is broad, there are many manufacturers, and the market competition is fierce. Therefore, improving the production process and reducing production costs are major issues facing the product. [0003] Oxytetracycline is produced by Streptomyces rimosus Sobinetal. At present, the breeding, preparation and preservation of strains produced by fermentation of oxytetracycline in domestic...

AI Technical Summary

Problems solved by technology

The disadvantage of the above process is that the yield of the strains will decrease during the slant passage and preservation process, the preservation period of the strains preserved in sand tubes is short, the mortality rate is high, and the strains are easy to degrade