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Hypsizigus marmoreus liquid strain culture medium and preparation method thereof

A technology of liquid strains and medium, which is applied in the field of liquid strain culture medium and its preparation of Shimeji mushroom, which can solve the problems of high pollution risk, long cultivation period of Shimeji mushroom, and labor-intensive, etc., so as to save energy and occupy The effect of reducing the risk of pollution due to the small area

Pending Publication Date: 2020-08-11
江苏华绿生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a liquid culture medium of shimeji mushroom and its preparation method, which can solve the problems of long cultivation period, labor cost and high risk of pollution in the existing shiji mushroom

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0020] Embodiment: a kind of jitake mushroom liquid culture medium and preparation method thereof, comprises the following steps:

[0021] S1 prepared medium: add 12g of glucose, 3g of peptone, 0.3g of magnesium sulfate, 0.3g of potassium dihydrogen phosphate, 0.4g of dipotassium hydrogen phosphate and 2.4g of soybean meal powder into 700ml of water to dissolve and stir evenly;

[0022] S2 adjusts the pH value to 6.0-6.6;

[0023] S3 Bottling Sterilization: According to the amount of liquid in each bottle is 70% of the volume of the culture bottle, put the prepared medium into the culture bottle, then seal it with a silica gel plug with air holes in the middle, and finally put it into the high pressure sterilization Keep it in the pot at 110-120°C for 20-30min to sterilize;

[0024] S4 Liquefaction treatment of solid strains: mash the solid strains in a sterile ultra-clean workbench, put them in sterile water, wash and let stand, and obtain the supernatant of the solid strain...

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PUM

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Abstract

The invention discloses a hypsizigus marmoreus liquid strain culture medium and a preparation method thereof. The preparation method comprises the following steps of S1, preparing a culture medium; S2, adjusting the pH value; S3, performing bottling and sterilizing; S4, carrying out liquefying on the solid strains; S5, performing inoculation; and S6, performing culturing. Through technical optimization, solid strains are converted into liquid strains for inoculation, the growth period of the strains is 55 days, the passage frequency of the strains is reduced by 5 generations through preparation of the liquid strains, the pollution risk in the strain passage process is reduced, the production labor is greatly reduced through the liquid strains, the occupied area is small, and more energy issaved.

Description

technical field [0001] The invention belongs to the technical field of fungi cultivation, and in particular relates to a liquid culture medium for mushrooms and a preparation method thereof. Background technique [0002] Since the 1980s, edible fungus fermentation technology has developed rapidly, and reports on the development of edible fungus submerged fermentation and small and medium-sized fermentation equipment are increasing. Shijiji mushroom is a kind of saprophytic fungus. Normal Shijiji mushroom strains are inoculated with solid strains. It takes 184 days from test tube to production and use. The growth cycle is long and the strains are passed on many times. It requires a lot of manual passage and inoculation operations. And every subculture operation increases the probability of strain contamination. Contents of the invention [0003] The purpose of the present invention is to provide a liquid culture medium of shimeji mushroom and its preparation method, which ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 冯占熊艳张建樟陈利娟滕磊刘琦李迁卞玲芝刘巧荣蒋来松毕欣李云余养朝
Owner 江苏华绿生物科技股份有限公司
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