Method for extracting isoflavone from soyabean molasses

A technology of soybean molasses and isoflavones, applied in the direction of organic chemistry, can solve the problems of different process equipment and methods, low G/D value of isoflavones, etc., and achieve the effect of less equipment investment, simple operation and reasonable process

Active Publication Date: 2010-09-08
山东万得福生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the different raw materials selected, the extraction process equipment and methods are also different, resulting in the low G / D value of the extracted isoflavones, which brings problems to the later development and application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Example 1, firstly, the raw material is selected as soybean molasses, a by-product when producing concentrated protein from low-temperature soybean meal, with an isoflavone content of 0.45% and a sugar content of 45. The process steps are: (1) use soybean molasses as the main raw material, pour it into a crystallization tank with stirring, add water to dilute to 30 Brix, heat the crystallization tank to boil for 6 minutes, (2) reduce the temperature of the molasses to Add glucono δ lactone at 75°C, the amount of glucono δ lactone added is 1‰ of the weight of molasses, continue to cool the molasses to 32°C and stop cooling, (3) stop stirring, and after 40 minutes of natural sedimentation, introduce centrifuge to separate In 10 minutes, (4) introduce the separated mother liquor into the storage tank, and the crystallized isoflavones are separated.

Embodiment 2

[0010] Example 2, firstly, the raw material is selected as soybean molasses, a by-product when producing concentrated protein from low-temperature soybean meal, with an isoflavone content of 0.55% and a sugar content of 50. The process steps are: (1) use soybean molasses as the main raw material, pour it into a crystallization tank with stirring, add water to dilute to a sugar content of 32, heat the crystallization tank to boil for 8 minutes, (2) reduce the temperature of the molasses to Add glucono δ lactone at 80°C, the amount of glucono δ lactone added is 1.5‰ of the weight of molasses, continue to cool the molasses to 30°C and stop cooling, (3) stop stirring, and after 38 minutes of natural sedimentation, introduce centrifuge to separate 15 minutes, (4) introduce the separated mother liquor into the storage tank, the crystallized isoflavones are separated, the separated isoflavones are diluted with warm water to a sugar content of 15, and then filtered with a 100-mesh siev...

Embodiment 3

[0011] Example 3, firstly, the choice of raw material is soybean molasses, a by-product when producing concentrated protein from low-temperature soybean meal, with an isoflavone content of 0.45% and a sugar content of 55. The process steps are: (1) use soybean molasses as the main raw material, pour it into a crystallization tank with stirring, add water to dilute to a sugar content of 28, heat the crystallization tank to boil for 3 minutes, (2) reduce the temperature of the molasses to Add glucono δ lactone at 79°C, the amount of glucono δ lactone added is 2‰ of the weight of molasses. When adding glucono δ lactone, stir and dissolve all glucono δ lactone with warm water first, then add to the crystallization tank During the process, continue to cool the molasses to 28°C and stop the cooling, (3) stop stirring, and after 35 minutes of natural sedimentation, introduce centrifuge for separation for 12 minutes, (4) introduce the separated mother liquor into the storage tank, and ...

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PUM

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Abstract

The invention relates to a method for extracting isoflavone from soyabean molasses. Firstly, a by-product-soyabean molasses which is obtained when concentrated protein is produced by using low-temperature soyabean meals is selected as a raw material, the isoflavone content of the soyabean molasses is not less than 0.22 percent, and sugar degree is 45-55. The method comprises the following process steps of: placing the soyabean molasses as the raw material into a crystallizing tank with stirring, adding water to dilute until the sugar degree is 26-33, and heating the crystallizing tank to increase the temperature till boiling, wherein boiling time is 3-8 minutes; (2) adding gluconic acid delta lactone when the temperature of the molasses is reduced to 70-85 DEG C, continuously reducing the temperature of the molasses to 25-35 DEG C, and then stopping reducing the temperature, wherein the adding amount of the gluconic acid delta lactone is 1-2 per thousand of the weight of the molasses; (3) stopping the stirring, naturally subsiding for 35-45 minutes, and then introducing to a centrifugal machine to separate for 10-15 minutes; and (4) leading out separated mother liquor so as to separate crystalline isoflavone. The invention has the raw material which is the by-product, small equipment investment and high cost performance of the extracted isoflavone.

Description

technical field [0001] The invention relates to a method for extracting isoflavones from soybean molasses in the technical field of biological material extraction. Background technique [0002] At present, there are mainly two kinds of raw materials used to extract isoflavones: one is to use molasses leached from soybean germ through alcohol aqueous solution to produce isoflavones; As a raw material of estrogen, when evaluating its pharmacological activity, it is not only the total isoflavone content, but the composition distribution is important, because the most effective active ingredient is genistin and its aglycones. The activity of G is higher than that of D (G is Genistin, and D is Daidzin). The higher the G, the better the product performance and the high quality of the product. With regard to the index analysis and comparison of the extraction of isoflavones from the aforementioned two raw materials, the isoflavone content of soybean germ (accounting for about 2% o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/36C07D311/40
Inventor 刘季善沙博
Owner 山东万得福生物科技有限公司
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