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Use of forsythiaside as synergist of antifungal agents

An antifungal drug, the technology of forsythiaside, which is applied in the field of medicine, can solve the problems of no reports of antifungal effects and no fungal diseases, and achieve the effects of saving medical expenses, improving antifungal effects, and reducing drug doses

Inactive Publication Date: 2010-09-01
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Studies have shown that forsythiaside has pharmacological effects such as antimicrobial, antiviral, antioxidative, elastase-inhibiting and α-interferon-inducing, but there is no report of its antifungal effect, let alone its increase as an antifungal drug. A report on the combined use of potentiator and antifungal drugs in the treatment of fungal diseases

Method used

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  • Use of forsythiaside as synergist of antifungal agents

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Example 1. The effect of the combination of forsythiaside and fluconazole on different clinical fungal strains

[0010] Materials and methods

[0011] 1. Test drug:

[0012] Forsythiaside: Shanghai Ronghe Pharmaceutical Technology Co., Ltd., batch number 090923.

[0013] Fluconazole injection: Pfizer Pharmaceutical Co., Ltd., batch number 9167802.

[0014] Dimethyl sulfoxide: Shanghai Shiyi Biotechnology Co., Ltd.

[0015] Forsythiaside was formulated with dimethyl sulfoxide at a concentration of 6.4 mg / ml.

[0016] All reagents were stored at -20°C. Before the experiment, the drug was taken out and placed in a 35°C incubator to melt, mixed well, and pharmacodynamic tests were carried out respectively.

[0017] 2. Strains:

[0018] Clinical strains: 1) Drug-resistant Candida albicans, including five strains 103, 904, 18, 38, and 0710448; 2) Candida tropicalis, including five strains 409, 936, 422, 0710453, and 0710087; 3) Cryptococcus neoformans, including 34880 ...

Embodiment 2

[0049] Example 2: Combination of forsythiaside and other azole antifungal drugs

[0050] Materials and methods

[0051] 1. Drugs:

[0052] Itraconazole, ketoconazole and miconazole were all provided by the School of Pharmacy, Second Military Medical University.

[0053] Itraconazole, ketoconazole and miconazole were prepared with dimethyl sulfoxide to prepare 6.4 mg / ml, and forsythiaside was prepared with dimethyl sulfoxide to prepare 6.4 mg / ml, and each reagent was stored at -20°C. Before the experiment, the drug was taken out and placed in a 35°C incubator to melt, mixed well, and pharmacodynamic tests were carried out respectively.

[0054] Other experimental steps and methods are the same as in Example 1, and the experimental results are shown in Tables 4, 5, and 6.

[0055]Table 4 MIC of forsythiaside and itraconazole (ITR) in combination with 4 strains of clinical drug-resistant Candida albicans 80 value

[0056]

[0057] Table 5 The MIC of the combination of for...

Embodiment 3

[0062] Example 3: The protective effect of forsythiaside combined with fluconazole on systemic fungal infection in mice

[0063] experimental method:

[0064] The mouse strain used in the experiment is a clean grade ICR female mouse (18-20g), certificate number: 2007000501825, provided by Shanghai Slack Experimental Animal Co., Ltd.

[0065] Drug-resistant Candida albicans (103 clinical drug-resistant strains), after the virulence recovery in mice, was reactivated on SDA medium, and grown in YEPD liquid medium to logarithmic growth phase. Adjust the bacterial concentration to 3.0×10 with normal saline 6 Each / ml, inject 0.1ml / 10g into the tail vein of mice according to body weight, and cause the model of deep fungal infection in mice. The mice in the normal control group were injected with 0.1ml / 10g normal saline through the tail vein.

[0066] 33 mice were randomly divided into 7 groups, except for 3 in the normal control group, and 5 in each group, which were fungal infect...

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Abstract

The invention relates to the technical field of medicines and discloses a new use of forsythiaside as a synergist of antifungal agents. In vivo and in vitro experiments show that the forsythiaside can be combined with the antifungal agents, such as fluconazole, itraconazole, miconazole, ketoconazole and the like, thereby improving the treatment effect of superficial and deep fungal infection with different degrees, leading the antifungal agents to recover the role to fungi with drug resistance and further using the forsythiaside as the synergist of the antifungal agents. The invention opens up the new use of the forsythiaside, and the use of the forsythiaside as the synergist of the antifungal agents can not only improve the antifungal role of drugs, but also lead the antifungal agents to recover the role to the fungi with drug resistance under the situations that the clinical drug resistance of the fungi is increasingly common and the drug resistance degree is increasingly serious, and further reduce the dosage of the antifungal agents, thereby saving medical expenses for patients and reducing toxicity and side effects of the drugs.

Description

technical field [0001] The invention relates to the technical field of medicine, and relates to a new application of forsythiaside as a synergist of antifungal drugs. Background technique [0002] Forsythiaside is one of the main active ingredients of traditional Chinese medicine Forsythia, and its chemical structure is as follows: [0003] [0004] Studies have shown that forsythiaside has pharmacological effects such as antimicrobial, antiviral, antioxidative, elastase-inhibiting and α-interferon-inducing, but there is no report of its antifungal effect, let alone its increase as an antifungal drug. Reports on the combined use of potentiating agents and antifungal drugs in the treatment of fungal diseases. Contents of the invention [0005] The invention provides a new application of forsythiaside as an antifungal drug synergist. In vitro cell experiments and animal experiments have shown that forsythiaside has obvious synergistic effects on antifungal drugs such as...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7032A61P31/10A61K31/4196A61K31/496A61K31/4174
Inventor 刘伟刘银丽曹永兵姜远英
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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