Test method of aphelenchoides ritzemabosi and diagnostic kit

Abstract

The invention discloses a test method of aphelenchoides ritzemabosi, and the method comprises the following steps: using SEQ ID No. 1 and SEQ ID No.2 in a sequence table as a primer pair, carrying out polymerase chain reaction, knowing whether a special fragment is obtained or not through electrophoresis and further detecting or identifying whether the special fragment is the aphelenchoides ritzemabosi or not. In addition, the invention further provides a kit for diagnosis of the aphelenchoides ritzemabosi, and the kit contains the primer pair. The method and the kit have very high reliability and sensitively, the operation is convenient without depending on the experiences of staff, the accuracy is high, and the practicality is strong, thereby meeting the requirements on rapid and reliable test and identification of the aphelenchoides ritzemabosi before planting crops and during the plant quarantine process.

Description

technical field [0001] The invention relates to a method for detecting or distinguishing the nematode chrysanthemum and a diagnostic kit capable of detecting the nematode chrysanthemum. Background technique [0002] Aphelenchoides ritzemabosi is one of the important plant parasitic nematode diseases of flowers. It has a wide range of hosts and can parasitize more than 200 kinds of plants such as ornamental plants, vegetables, small fruits and weeds. The nematode primarily damages leaves, but can also infect flower buds and flowers. In addition to harming chrysanthemums, this nematode also harms asters, coneflowers, dahlias, zinnias and other Compositae plants, as well as Solanaceae plants or Poppyaceae plants, or tobacco, strawberry, pearl orchid, lily, and cattail flowers. , delphinium, oleander and other crops or flowers. The leaves of the damaged plant are withered, the flowering is not good or cannot be bloomed, and in severe cases, the whole plant turns black, and eve...

AI Technical Summary

Problems solved by technology

There are certain disadvantages in this method, which are mainly manifested in: 1. The chrysanthemum chrysanthemum chrysanthemum belongs to the genus chrysanthemum genus, and there are many kinds of chrysanthemum nematodes. Usually, there will be a variety of nematodes with similar shapes in the samples identified, which requires the appraiser to have Rich experience; 2. There are differences among individual nematodes, which requires sufficient identification samples; 3. Morphological identification is only applicable to adults, but not to larvae

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