Conductive polymer for biosensor and preparation method thereof

Abstract

The invention provides a conductive polymer for a biosensor and a preparation method thereof in order to solve the problem puzzling the academia for long time about how to combine a bioactive unit to a metal non-inactively and permanently and that bioactive molecules are connected to the conductive polymer in one step. The bioactive unit is first introduced into a conductive polymer monomer, and then the conductive polymer with bioactivity is directly formed in situ on the surface of an electrode in one step by adopting an in-situ electrochemical combinatorial synthesis method and an independent addressing microelectrode array technique so as to obtain a functional microelectrode array applied to the biosensor. The conductive polymer for the biosensor and the preparation method make the development of the biosensor towards the direction of intensification, microminiaturization and micro power consumption become possible, can promote the development of disease diagnosis, medicament screening, health care, judicial expertise, food detection, environmental monitoring, individualized medicament and the like and industrial chains thereof, and have far-reaching social meaning and broad market value.

Description

technical field The present invention relates to a kind of conductive polymer used in biosensors and its preparation method, in particular, relates to a method of in-situ electrochemical combination synthesis (combinatorial synthesis) to make polymers with bioactive units (such as DNA, RNA, Conductive polymers of peptides, proteins, enzymes, aptamers (aptamers, tissues, cells, etc.) are directly immobilized on electrodes in one step to obtain functionalized microelectrode arrays for application in biosensors. Background technique Biosensor is a high-tech developed by the interpenetration of various disciplines such as bioinformatics, biocybernetics, bionics, biocomputer, biology, chemistry, physics, medicine, and electronic technology. A rapid, parallel, microanalysis method. The substance to be measured flows through the biosensor, and through molecular recognition, the signal expressed by the interactive reaction between the biologically active units is converted into a q...

AI Technical Summary

Problems solved by technology

The absence of conventional equipment to fabricate stable and reproducible conductive polymer biosensors has largely limited the wide application of such sensors in the field of analysis.

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