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House lizard glycopeptide, method for preparing same and medicinal application thereof

A technology of geckoglucose and geckoglucose, applied in the field of geckopeptide and its preparation, can solve problems such as limitation of drug dosage, toxicity, and influence on therapeutic effect, and achieve the effect of inhibiting tumor cell migration and improving biological activity

Active Publication Date: 2010-07-07
天津众妙之门科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Generally speaking, most traditional Chinese medicines for animals have certain toxicity to the human body, which limits the dosage of such medicines during treatment and greatly affects the therapeutic effect

Method used

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  • House lizard glycopeptide, method for preparing same and medicinal application thereof
  • House lizard glycopeptide, method for preparing same and medicinal application thereof
  • House lizard glycopeptide, method for preparing same and medicinal application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Geckoglycopeptide, characterized in that: the weight average molecular weight is greater than 2,000,000Da; the specific rotation is -60°; the mass percentage of carbon element is 23.39%, the mass percentage of hydrogen element is 4.925%, and the mass percentage of nitrogen element The percentage content is 6.51%; the sugar part of its composition contains rhamnose, fucose, mannose, glucose, galactose, N-acetylglucosamine, and the molar ratio is: rhamnose: fucose: mannose : Glucose: Galactose: N-acetylglucosamine = 1.334: 3.363: 3.375: 6.983: 10.480: 0.019; the connection mode of monosaccharides is: 1,3-linked rhamnose, T-linked fucose, 1,2,3,6-,1,3,4-linked mannose, T-,1,4-linked glucose, T-,1,4-linked galactose and 1,4-linked N-acetylglucosamine; the mass percentage of uronic acid is 3.08%; its binding protein part contains 16 kinds of amino acids, and the mass percentage is 16.33%; the type of glycopeptide bond contains O-linkage; The mass percentage is 14.7%.

[00...

Embodiment 2

[0043] Preparation of gecko glycopeptide: Take 1000g of the whole gecko, cut into 0.2cm long pieces, soak in 6000ml, 3000ml of methanol for 2h, filter, put the dregs in a ventilated place at room temperature to dry, and then use 8000ml, 6000ml , 4000ml of hot water was extracted three times at 100°C, and the extraction time was 6h, 4h, and 3h respectively. Combine the extracts, filter, concentrate to 800ml, add ethanol precipitation with a final concentration of 80% (V / V), centrifuge (3200r / min, 10min), collect the precipitate, redissolve the precipitate in water, centrifuge (3200r / min, 10min ), take the supernatant, remove free protein 10 times by conventional sevage method, concentrate, dialyze, concentrate, and dry. Take dry powder 24g and dissolve in 1200ml containing 0.5mol / L sodium chloride, 10mmol / L cysteine, 0.1mol / L phosphate buffer (pH6.5) of 10mmol / L EDTA, add 120mg Papain and 10ml of toluene, 60 ° C water bath for 48 hours, then add 60 mg of papain, continue to 60...

Embodiment 3

[0045] Preparation of Gecko Glycopeptide: Take 1000g of the whole Gecko, crush it into coarse powder, pass through a No. 1 sieve, soak in 3000ml of methanol for 12h, filter, put the dregs in a ventilated place at room temperature to dry, and then use 10000ml of hot water at 60°C Extract three times, the extraction time is 6h. Combine the extracts, filter, concentrate to 1000ml, add ethanol with a final concentration of 70% (V / V) to precipitate, stand at 4°C for 24h, centrifuge (4000r / min, 6min), collect the precipitate, redissolve the precipitate in water, and centrifuge (4000r / min, 6min), take the supernatant, dialyze and dry. Take dry powder 24g and dissolve in 4200ml containing 0.5mol / L sodium chloride, 10mmol / L cysteine, 0.1mol / L phosphate buffer (pH6.5) of 10mmol / L EDTA, add 480mg Papain and 40ml toluene, 70°C water bath for 8h, then add 240mg of papain, continue 70°C water bath for 4h. The enzymolysis solution was filtered, concentrated under reduced pressure to 500ml,...

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Abstract

The invention relates to house lizard glycopeptide, a method for preparing the same and medicinal application thereof. The weight average molecular weight of the house lizard glycopeptide is more than 2,000,000 Da; the house lizard glycopeptide contains rhamnose, fucose, seminose, glucose, galactose, N-acetyl-D-glucosamine and uronic acid; the binding type protein of the house lizard glycopeptidecontains 16 amino acids which account for 16.33 percent of the total mass of the house lizard glycopeptide; and sulfate accounts for 14.7 percent of the total mass of the house lizard glycopeptide. Through further research on the basis of the application of house lizard polysaccharide, a scientific test result proves that the house lizard glycopeptide has higher curative effect on malignant tumors compared with that of the house lizard polysaccharide, can effectively inhibit the multiplication and migration of tumor cells, can be independently used for the treatment of the malignant tumors, auxiliary treatment, such as tumor operations, radiotherapy, chemotherapy and transcatheter embolization, and the prevention of viral infectious diseases, such as aids and the like, and also can be used as a novel immunomodulator used for treating diseases, such as tumors, chronic infection and the like.

Description

technical field [0001] The invention relates to an active ingredient with high medicinal value in animal Chinese medicinal materials, a preparation method and its medical application, in particular to a geckoglycopeptide, its preparation method and its medical application. Background technique [0002] Traditional Chinese medicine is the quintessence of our country. Chinese medicinal materials are the material treasure house of traditional Chinese medicine theory. Chinese medicinal materials are divided into plant Chinese medicinal materials, animal Chinese medicinal materials and mineral Chinese medicinal materials. One of the major features, such as extracting cantharidin and cantharidinate from mylabris, has achieved certain effects in the treatment of malignant tumors, and also has a certain antiviral effect. Generally speaking, most traditional Chinese medicines for animals have certain toxicity to the human body, which limits the dosage of such medicines during treatme...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/435C12P21/06A61K38/14A61P35/00A61P1/16A61P31/12A61P31/18A61P37/02
Inventor 吴雄志
Owner 天津众妙之门科技有限公司
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