Method for preparing high-ferric content ferroheme polypeptide composite with pepsin hydrolysis method

A technology of heme polypeptide and protease, which is applied in the field of bioengineering, can solve the problem of heme products being insoluble in acidic aqueous solution, etc., and achieve the effects of high content of heme iron, reduced content, and easy digestion and absorption

Active Publication Date: 2010-06-23
长春白求恩制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The invention provides a heme polypeptide compound with high iron content prepared by protease hydrolysis, which over

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Take 10kg of fresh pig blood and add an equal amount of water for hemolysis, heat to boiling, cool to 40 degrees Celsius, add 20 grams of papain, and stir for 20 hours at 40 degrees Celsius. Stop heating and keep warm, collect slurry sediment by high-speed centrifugation, dry and crush to obtain the first hydrolysate. Then add twice the weight of water and stir, then add 20 grams of papain, and keep stirring at 40 degrees Celsius for 20 hours. Stop heating and keep warm, collect the slurry-like sediment by high-speed centrifugation, dry and pulverize to obtain the second hydrolysis product of heme polypeptide with higher iron content.

[0018]

Embodiment 2

[0020] Take 10kg of fresh pig blood and add equal amount of water to hemolyze, heat to boiling, cool to 40 degrees Celsius, add 40 grams of papain, and stir at 40 degrees Celsius for 20 hours. Stop heating and keep warm, collect slurry sediment by high-speed centrifugation, dry and crush to obtain the first hydrolysate. Then add twice the weight of water and stir, then add 40 grams of papain and keep stirring at 40 degrees Celsius for 20 hours. Stop heating and keep warm, collect slurry sediment by high-speed centrifugation, dry and pulverize, to obtain heme polypeptide powder with higher iron content.

[0021]

Embodiment 3

[0023] Take 10kg of fresh pig blood and add an equal amount of water for hemolysis, heat to boiling, cool to 40 degrees Celsius, add 10 grams of papain, and stir for 20 hours at 40 degrees Celsius. Stop heating and keep warm, collect slurry sediment by high-speed centrifugation, dry and crush to obtain the first hydrolysate. Then add twice the weight of water and stir, then add 10 grams of papain and keep stirring at 40 degrees Celsius for 20 hours. Stop heating and keep warm, collect slurry sediment by high-speed centrifugation, dry and pulverize, to obtain heme polypeptide powder with higher iron content.

[0024]

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Abstract

The invention discloses a method for preparing high-ferric content ferroheme polypeptide composite with pepsin two-hydrolysis method, which is a method for preparing ferroheme polypeptide with twice enzyme hydrolysis of blood, and polypeptide generated by hydrolyzed protein wraps ferroheme to form frroheme polypeptide composite; adopting twice protease hydrolysis of hemoglobin, ferroheme combineswith peptide generated by proteolysis to generate composite (ferroheme polypeptide) of ferroheme and polypeptide, the combination of the composite is very firm, and is not easy to separate, the enzyme hydrolysis can realize the further dissociation of peptide in ferroheme polypeptide, the ferroheme content in ferroheme polypeptide is improved, the polypeptide content thereof is reduced, and heme iron content is high; since ferroheme has the combination and coverage of hydrophilic polypeptide, the composite has a certain hydrophilcity, is easily solute in acid solution such as gastric acid, acetic acid and the like and is easy to digest and absorb.

Description

Technical field [0001] The present invention relates to a method for heme polypeptide complexes, and in particular provides a hydrolysis method for preparing heme polypeptide complexes with high iron content by protease hydrolysis, which belongs to the technical field of bioengineering. Background technique [0002] Heme is an iron-containing porphyrin (iron porphyrin) compound and an important component of hemoglobin contained in red blood cells. Hemoglobin is a binding protein of heme, and its heme is bound to histidine residues (ligands) of globin through iron atoms through a coordinate bond to form a binding protein. [0003] Heme can be directly absorbed by the small intestine and is not affected by the substances contained in plant foods that hinder the absorption of iron ions. Its absorption and utilization rate is much higher than that of inorganic iron salts and iron ions contained in plant foods. Animal blood is a rich source of hemoglobin, but the protein must be digest...

Claims

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Application Information

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IPC IPC(8): C12P21/06
Inventor 麦荫乔岳海涛王友联谢海泉
Owner 长春白求恩制药有限公司
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