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Mycelium concentration method

A cell-volume ratio technology, applied in the field of applied microorganisms, can solve the problems of low molecular weight and high manufacturing cost, and achieve the effects of low cost, good safety and simple process flow.

Inactive Publication Date: 2012-09-05
无锡绿水之源生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although polyelectrolyte flocculants with high charge density and high molecular weight have a good effect on the flocculation of bacterial fermentation broth, the manufacturing cost of such flocculants is relatively high at present, and the molecular weight of domestic polyelectrolyte flocculants is generally low, so they cannot Good flocculation and concentration effect can be achieved within the allowable range of cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1: The cultured Pseudomonas stutzeri (Pseudomonas stutzeri) fermentation broth was used as the solution to be tested. Prepare the fermentation broth following the cultivation method mentioned in Methods.

[0036] Use the compound flocculation material A to conduct the cell concentration experiment, the flocculant addition ratio is 0.2%~2%, the pH is adjusted to 4~5, the stirring time is 10-30 minutes at 100~300rpm, and then the natural precipitation is 10-24 hours, and the flocculation is observed Precipitation effect.

[0037] Measure the floc volume with a graduated cylinder, take the supernatant, and measure the absorbance at a wavelength of 600nm; use the centrifuged fermentation broth (8000r / min, centrifuged for 30min) as a blank control a, and measure the absorbance at a wavelength of 600nm; The stock solution was control b, and the absorbance was measured at a wavelength of 600nm; the change in OD (optical density) value was used as an index to measure t...

Embodiment 2

[0047] Example 2: The cultured Pseudomonas stutzeri fermented liquid was used as the solution to be tested. Prepare the fermentation broth following the cultivation method mentioned in Methods.

[0048] The bacteria concentration experiment was carried out with the compound flocculation material B. The flocculant addition ratio was 0.2%-2%, the pH was adjusted to 4-5, the stirring time was 10-30 minutes at 100-300rpm, and then the natural precipitation was 10-24 hours, and the flocculation was observed Precipitation effect.

[0049] Use a graduated cylinder to measure the volume of the flocs, and take the supernatant, use the centrifuged fermentation broth (8000r / min, centrifuged for 30min) as the blank control a, and the fermentation broth stock solution as the control b, measure the absorbance at a wavelength of 600nm, and use the fermentation broth The change of OD (optical density) value is used as an index to measure the concentration of bacteria after flocculation, and ...

Embodiment 3

[0054] Example 3: The cultured Pseudomonas stutzeri fermented liquid was used as the solution to be tested. Prepare the fermentation broth following the cultivation method mentioned in Methods.

[0055] Bacteria concentration with a single flocculation substance: add the above-mentioned first flocculation substance with a mass volume ratio of 0.1% to 1% to 100 to 500mL of microbial fermentation broth, stir at 100 to 300rpm at 20-25°C for 5 to 20 Minutes, then add the second flocculation material with a mass volume ratio of 0.1% to 1%, stir at 100 to 300rpm at 20-25°C for 5 to 20 minutes, and then add the third flocculation material with a mass volume ratio of 0.1% to 1%. Stir at 100-300rpm at 20-25°C for 5-20 minutes, and let stand naturally for 10-24 hours to observe the effect of flocculation and precipitation.

[0056] Use a graduated cylinder to measure the volume of the flocs, and take the supernatant, use the centrifuged fermentation broth (8000r / min, centrifuged for 30...

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Abstract

The invention relates to a simple and easy mycelium concentration method, which belongs to the technical field of applied microbiology. Sodium carboxymethyl cellulose, cationic polyacrylamide and ammonium citrate tribasic are taken as flocculation substances; the concentration uses the single flocculation substance or one of the complex flocculation substances of the substances, the assembly is carried out according to a certain proportion, then a certain amount of the flocculation substance is taken and added in microbial fermentation broth which completes the culture, the action is carried out for a certain time, and mycelia in the fermentation broth is flocculated and further precipitated for achieving the concentration effect. Reagents used by the method are chemical substances without toxicity or side effects, the mycelia obtained by flocculation can not only keep the original activity, but also avoid causing harms when being applied in water quality improvement of a water body and other fields, thereby being an environment-friendly product. Only the supernatant liquid needs to be sucked after the precipitation of suspended micro-organisms, thereby having the advantages of good safety, low cost, simple entire process and high efficiency.

Description

technical field [0001] The invention discloses a simple and easy cell concentration method, relates to a cell concentration method for microbial preparations used in industry, agriculture or aquaculture, and belongs to the field of applied microorganism technology. Background technique [0002] With the popularization of the application of microorganisms in all walks of life and the continuous warming up year by year, people's research on the practical application of beneficial microorganisms not only focuses on screening or constructing excellent strains, but also includes different degrees of fermentation of the bacteria obtained from fermentation. Concentration, thereby reducing transportation, storage and use costs, and improving the effect of effective microorganisms per unit concentration. For most microbial fermentation broths, because the bacteria in the fermentation broth are small and the components are complex, the flocculation and concentration load is heavy, and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/00C12N1/20C12N1/16C12R1/38C12R1/125C12R1/72
Inventor 王小娟许骄阳
Owner 无锡绿水之源生物科技有限公司
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