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Method for continuously extracting functional components of chlorella vulgaris

A functional component, chlorella technology, applied in the preparation method of peptides, chemical instruments and methods, algae/bryopeptides, etc., can solve the problems of unclear products, long operation cycle, low yield, etc., and achieve simple operation Reliable, easy-to-maintain active effect

Inactive Publication Date: 2010-06-16
BOHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] CN1018071B has introduced a kind of ecological harvesting method of chlorella, but the operation cycle is too long, the yield is low, and affects the normal growth of chlorella
CN1302013C introduces a preparation method of active chlorella polysaccharides, which breaks the wall by ultrasonic waves, and then extracts and removes impurities by hot leaching. functions such as cerebrovascular diseases, but its yield is low, and other functional components of chlorella are wasted
CN101053577A discloses the preparation method of chlorella pressure broken wall and nucleotide, protein, polysaccharide, algae dry powder, adopts normal temperature pressure broken wall to chlorella, but needs - 20 ℃ freezing in the process of preparing product, and its energy consumption very high
There is also the phenomenon that the product is not clear, the yield is low and the waste is serious

Method used

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  • Method for continuously extracting functional components of chlorella vulgaris
  • Method for continuously extracting functional components of chlorella vulgaris

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Use a pump to introduce the chlorella culture solution into a membrane separation system with polysulfone (or ceramic or polyether) as the membrane material for microporous membrane filtration. The control membrane separation process parameters are: the cross-flow velocity is 2.4cm / s, the operating pressure The temperature is 0.02MPa, the temperature is 20°C, the chlorella is concentrated to 0.5% of the original volume, and the chlorella concentrate is obtained. Add 0.1% of cellulase, 0.1% of pectinase, and 0.1% of xylanase to the concentrated solution (the amount of enzyme added is the percentage of dry matter in the concentrated solution of Chlorella), at a temperature of 40°C, and carry out enzymatic hydrolysis for 1 hour Broken. To obtain the concentrated liquid of broken chlorella, ethanol was added to the concentrated liquid of broken chlorella to make the concentration 50%, the temperature was 50° C., and extraction was carried out for 2 hours. Then centrifuge (...

Embodiment 2

[0035]Use a pump to introduce the chlorella culture solution into a membrane separation system using polysulfone (or ceramics or polyether) as a membrane material for microporous membrane filtration. The control membrane separation process parameters are: the cross-flow velocity is 5cm / s, and the operating pressure is 0.04MPa, the temperature is 30°C, the chlorella is concentrated to 0.8% of the original volume, and the chlorella concentrate is obtained. Add 0.2% of cellulase, 0.3% of pectinase, and 0.4% of xylanase to the concentrated solution (the amount of enzyme added is the percentage of dry matter in the concentrated solution of Chlorella), at a temperature of 50°C, and carry out enzymatic hydrolysis for 2 hours The wall is broken to obtain the concentrated solution of the broken wall chlorella. Add ethanol to the concentrated solution of broken chlorella to make the concentration 60%, the temperature is 55° C., and extract for 3 hours. Then centrifuge (3000r / min) to ob...

Embodiment 3

[0037] Use a pump to introduce the chlorella culture solution into a membrane separation system using polysulfone (or ceramics or polyether) as a membrane material for microporous membrane filtration. The control membrane separation process parameters are: the cross-flow velocity is 4cm / s, and the operating pressure is 0.08MPa, the temperature is 40 ℃, the chlorella is concentrated to 1% of the original volume, and the chlorella concentrate is obtained. Add 0.3% of cellulase, 0.3% of pectinase, and 0.3% of xylanase to the concentrated solution (the amount of enzyme added is the percentage of dry matter in the concentrated solution of Chlorella), at a temperature of 60°C, and carry out enzymatic hydrolysis for 5 hours The wall is broken to obtain the concentrated solution of the broken wall chlorella. Add ethanol to the broken chlorella concentrate to make the concentration 70%, the temperature is 50°C, and extract for 6 hours. Then centrifuge (3000r / min) to obtain ethanol ext...

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Abstract

The invention relates to a method for continuously extracting functional components of chlorella vulgaris, which comprises the steps of enriching the chlorella vulgaris through the membrane separation technology, obtaining concentrated slurry of the chlorella vulgaris, adding a proper enzyme in a liquid phase system for wall-breaking, and using a solvent for extracting chlorophyll from chlorella vulgaris solution after wall-breaking, thereby being capable of obtaining a functional pigment product and reducing the color value of follow-up products; and firstly carrying out enzymatic hydrolysis on a water phase of the chlorella vulgaris after decoloring, then using the solvent for extracting functional grease, extracting active polysaccharides from the obtained degreased chlorella vulgaris by using the acid method, and then drying the other parts for obtaining crude proteins of the chlorella vulgaris. The main products comprise the chlorophyll, the active polysaccharides of the chlorella vulgaris, the functional grease of the chlorella vulgaris, the proteins of the chlorella vulgaris and the like, and the method optimizes the process on the basis of ensuring the activity of the functional components of the chlorella vulgaris, thereby obtaining high yield and reducing production cost.

Description

technical field [0001] The invention belongs to the technical field of marine organisms, in particular to a method for continuously extracting functional components of chlorella. Background technique [0002] Chlorella is a universal single-celled green alga, belonging to the genus Chlorella of the Oocystaceae Chlorella of the Chlorophyceae Chlorophyceae. It is widely distributed in nature and can use light energy to support itself and also use organic carbon sources under heterotrophic conditions. To grow and reproduce. Now there are about 15 species of chlorella in the world, and there are hundreds of variants. Chlorella is rich in comprehensive nutrients and biologically active substances. Chlorella usually has a high protein content (55%-67%), contains more sugars (15%-20%) and lipids (5%-10%), and contains biologically active polysaccharides, growth factors, and glycoproteins. , glycolipid protein, dietary fiber, etc. It contains a complete range of amino acids in t...

Claims

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Application Information

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IPC IPC(8): C12P7/64C09B61/00C08B37/00C07K14/405C07K1/14
Inventor 刘贺朱丹实何余堂郭晓飞刘昊东
Owner BOHAI UNIV
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