Method for separating and purifying oligodendrocyte precursor cells
A precursor cell, separation and purification technology, applied in the field of separation and purification of oligodendrocyte precursor cells, can solve the problems of low acquisition rate of OPCs, cell death, long time consumption, etc., and achieve good proliferation growth and directional differentiation ability, High-efficiency, high-purity, actionable effects
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[0026] Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings.
[0027] 1. Experimental materials
[0028] Clean-grade SD rats born 0 to 3 days old were purchased from the Animal Center of the Third Military Medical University of the Chinese People’s Liberation Army. The rats were anesthetized by burying them in ice for 5 minutes, their heads were wiped with alcohol, and their heads were decapitated. Bacteria were washed twice in PBS to remove blood, and then placed in sterile D-Hank's solution containing penicillin at a concentration of 160 U / ml and streptomycin at a concentration of 200 U / ml at a temperature of 4°C, and stripped under a dissecting microscope. Brain, spare.
[0029] Trypsin was purchased from Boster Company, DNase was purchased from Shanghai Sangon Bioengineering Technology Service Co., Ltd., Poly-D-Lysine (Poly-D-Lysine), insulin and triiodothyronine (Triiodothyronine) were purchas...
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