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Method for quickly detecting melamine in milk sample based on nanogold

A technology of melamine and nano-gold, which is applied in the preparation of test samples, measurement of color/spectral characteristics, and material analysis by observing the impact on chemical indicators, etc., which can solve the problem of high detection operation requirements, complex detection process, and detection cost. Advanced problems, to achieve the effect of simple and feasible preparation method, low detection cost and good stability

Inactive Publication Date: 2010-06-02
FUZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Solve the problems in the prior art that the detection operation requirements for melamine are high, the detection process is complicated, the detection cost is high, the detection time is long, and the requirements for a large number of food inspections cannot be met. The method of the present invention is low in cost, easy to operate, and can be visualized and quickly detected. Melamine in milk

Method used

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  • Method for quickly detecting melamine in milk sample based on nanogold
  • Method for quickly detecting melamine in milk sample based on nanogold
  • Method for quickly detecting melamine in milk sample based on nanogold

Examples

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Effect test

preparation example Construction

[0041] 1. Preparation of 13nm gold nanoparticles

[0042] Using the method of reducing chloroauric acid with sodium citrate, heat 50mL of 1mM chloroauric acid solution to boiling, then quickly add 5mL of 38.8mM trisodium citrate, after reflux reaction for 15min, stop heating, and naturally cool to room temperature under stirring , the maximum absorption wavelength of the gold nanometer prepared by this method is 520nm, and the color of the solution is wine red.

[0043] 2. The application of gold nanoparticles to the detection of melamine

Embodiment 1

[0044] Embodiment 1 Nano gold detects melamine in liquid milk

[0045] Weigh 11 parts of 1.6g liquid milk containing 0ppm, 0.48ppm, 0.95ppm, 1.90ppm, 3.80ppm, 5.70ppm, 7.60ppm, 9.50ppm, 11.4ppm, 13.3ppm, 15.2ppm melamine respectively into a 7ml centrifuge tube, add 0.72mL trichloroacetic acid (10%) and 1mL chloroform Trichloroacetic acid and chloroform, centrifugal separation after ultrasonic treatment (13000rpm centrifugation 6 minutes after 200W ultrasonic treatment 15 minutes) remove protein and fat, the supernatant obtained is washed with 1M sodium carbonate solution Adjust its pH to pH 8.0, then further remove the precipitate by centrifugation (3000rpm centrifugation for 1min), take the supernatant and carry out melamine detection according to the following method: take 0.1mL nano gold solution, add 0.2mL different melamine mass ratio The liquid milk supernatant, mixed evenly, placed for 0.5min after the color reaction, carried out UV-visible absorption spectrum analysis ...

Embodiment 2

[0046] Embodiment 2 Nano-gold detects melamine in milk powder

[0047] Weigh 13 parts of 0.6g milk powder containing 0ppm, 0.84ppm, 1.93ppm, 4.2ppm, 12.6ppm, 21ppm, 29.4ppm, 37.8ppm, 46.2ppm, 54.6ppm, 63ppm, 71.4ppm, 79.8ppm of melamine to a 7ml centrifuge tube , add 2 mL of secondary water to dissolve, add 2 mL of trichloroacetic acid (10%) and 1.2 mL of chloroform, centrifuge after ultrasonic treatment (200W ultrasonic treatment for 15 minutes and then centrifuge at 13,000 rpm for 6 minutes) to remove protein and fat, and the supernatant The pH of the liquid was adjusted to pH 7.0 with 1M sodium carbonate solution, and then the precipitate was further removed by centrifugation (centrifugation at 3000rpm for 1min), and the supernatant was taken for melamine detection according to the following method: take 0.1mL of nano-gold solution, Add 0.2mL of milk powder supernatant with different mass ratios of melamine, mix evenly, place for 0.5min for color reaction, and then conduct ...

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Abstract

The invention provides a method for quickly detecting melamine in a milk sample based on nanogold, which aims to solve the problems of the high operation requirement, complex detection process, high cost, long time, incapacity of meeting a lot of food test requirements and the like on the detection of the melamine. The method comprises the following steps: performing pretreatment on the milk sample and adding the pretreated milk sample into a nanogold solution; and adopting visual colorimetry to compare the color of the nanogold added with the sample solution with the color in a color standard series and then qualitatively judging whether the milk contains the melamine or not. The color standard series is prepared through the following steps: adding the pretreated milk samples in an identical volume and containing the melamine of different concentrations into the nanogold solution, wherein the content of the nanogold in each sample is consistent; and after color development reaction, obtaining the color standard series. The method has the advantages of low cost, simple and convenient operation and capacity of visually and quickly detecting the melamine in the milk.

Description

technical field [0001] The invention belongs to the technical field of melamine detection, and more specifically relates to a method for rapidly detecting melamine in milk samples based on nano-gold. Background technique [0002] Melamine is a triazine nitrogen-containing heterocyclic organic compound, referred to as triamine, commonly known as melamine and protein essence. As a widely used organic chemical raw material, melamine is mainly used as a raw material for the production of melamine formaldehyde resin (MF). In addition, melamine is also used as a flame retardant, water reducer, formaldehyde cleaner, etc. Since the food industry mainly uses the Kjeldahl method to determine the content of nitrogen atoms to indirectly calculate the protein content in food, in recent years, melamine has been added to food and feed by criminals because of its high nitrogen content (66%) to increase The apparent protein content of the product. Studies have shown that melamine is slight...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N21/33G01N1/34
Inventor 郭良洽钟坚海
Owner FUZHOU UNIVERSITY
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