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Penicillium and application thereof in catalyzing and hydrolyzing lignocellulose

A technology of lignocellulose and Penicillium, applied in the field of cellulase and xylanase to catalyze the hydrolysis of lignocellulose, to achieve good catalytic effect, good industrial application and development prospects, and easy preparation

Inactive Publication Date: 2010-06-02
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are relatively few reports on Penicillium sp. that can simultaneously produce high cellulase and xylanase, and the general activity is lower than that of enzymes produced by Trichodema sp.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The screening of embodiment 1 bacterial strain

[0027] Screening medium formula: 60 mesh corn cob 10.0g / L, yeast extract 4.5g / L, NH 4 NO 3 3.0g / L, NaCl 5.0g / L, K 2 HPO 4 2.0g / L, MgSO 4 ·7H 2 O 0.4g / L, agar powder 15.0g / L, pH 7.0.

[0028] Shaking tube viability test medium formula: 60 mesh corn cob 10.0g / L, NaNO 3 2.0g / L, peptone 1.0g / L, K 2 HPO 4 2.0g / L, KH 2 PO 4 3.0g / L, MgSO 4 ·7H 2 O 0.5g / L, anhydrous CaCl 2 0.1g / L, Mandels trace element solution 1.0ml / L, pH 5.0.

[0029] Rich medium formula: glucose 20.0g / L, peptone 3.0g / L, yeast extract 0.5g / L, KH 2 PO 4 2.0g / L, (NH 4 ) 2 SO 4 1.5g / L, anhydrous MgSO 4 0.15g / L, anhydrous CaCl 2 0.3g / L, Mandels trace element solution 1.0ml / L, pH 5.0.

[0030] Take a small amount of soil sample and suspend it in 3ml of sterile water, dilute to 10 -4 , spread the suspension on a screening medium plate with corncob as the only carbon source, and culture it statically at 30°C for 1 to 2 days, and then pick a singl...

Embodiment 2

[0031] The cultivation of embodiment 2 microorganisms

[0032] Seed medium formula: glucose 20.0g / L, peptone 3.0g / L, yeast extract 0.5g / L, KH 2 PO 4 2.0g / L, (NH 4 ) 2 SO 4 1.5g / L, anhydrous MgSO 4 0.15g / L, anhydrous CaCl 2 0.3g / L, Mandels trace element solution 1.0ml / L, pH 5.0.

[0033] Fermentation medium formula: 60 mesh corn cob 30.0g / L, NaNO 3 10.0g / L, KH 2 PO 4 2.0g / L, MgSO 4 0.15g / L, CaCl 20.3g / L, Mandels trace element solution 1.0ml / L, Tween-80 5.0g / L, pH 6.0.

[0034] Take the Penicillium slant preserved at 4°C, pick a ring and inoculate it into a 250ml shake flask containing 50ml seed culture medium. Cultivate at 30°C for 24 hours at 180rpm, transfer to a 500ml shaker flask containing 100ml of fermentation medium at an inoculum size of 15% (v / v), cultivate at 30°C and 180rpm for 5 days, and centrifuge to obtain the fermentation supernatant liquid. The measured enzyme activity of the fermentation broth was 2.4 FPU / ml for cellulase and 241 IU / ml for xylana...

Embodiment 3

[0037] Example 3 Catalytic hydrolysis of corn stalks (20ml scale) using Penicillium crude enzyme solution

[0038] In 20ml of citric acid buffer (50mM, pH 4.8), add 0.4g of steam explosion pretreated corn stover (containing 32.6% cellulose, 26.4% hemicellulose), and then add the fiber produced by Penicillium sp.ECU0913 Sulfase 2.61FPU, make its addition be 20FPU / g dextran, after reacting 72h under the condition of 50 ℃, 200rpm, detect the content of glucose, xylose in the reaction liquid with HPLC (Adopting the Aminex HPX-87P chromatography of Bio-Rad Company Column, φ0.78cm×30cm, mobile phase is ultrapure water, flow rate 0.4ml / min, column temperature 85°C). The concentrations of glucose and xylose in the reaction liquid were measured to be 3.5 g / L and 0.97 g / L, respectively. The hydrolysis conversion rates of cellulose and hemicellulose were calculated to be 48% and 25%, respectively.

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Abstract

The invention discloses Penicillium sp.ECU0913 capable of degrading biomass and with preservation number of CGMCC No.3371 and application thereof. By using a Penicillium strain, cellulose and xylanase can be obtained by simultaneous fermentation, the biocatalysis efficacy can be exerted, biomass rich in cellulose and hemicellulose can be decomposed and monosaccharide can be generated; and the obtained hydrolysate which can be used as a carbon source is used for synthesizing a biological energy source and bio-based chemical products.

Description

technical field [0001] The invention belongs to the field of biocatalysis, and relates to a penicillium strain and a method for catalyzing the hydrolysis of lignocellulose by using cellulase and xylanase produced by fermentation of the strain. technical background [0002] With the increasing depletion of global oil reserves, the shortage of liquid fuels will seriously restrict the development of human beings. Finding renewable alternative resources has become an urgent task to maintain the sustainable development of human society. Vigorously developing new renewable resources is also a prerequisite for China to maintain sustainable development in the 21st century. The production of bioenergy and bio-based chemicals using the most abundant renewable resource on earth—plant cellulose as a raw material has attracted widespread attention at home and abroad, and is considered to be one of the best ways to deal with the depletion of fossil resources in the 21st century. Cellulos...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P19/02C12R1/80
Inventor 郁惠蕾石乾乾李春秀许建和
Owner EAST CHINA UNIV OF SCI & TECH
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