Preparation method of high-efficiency compound microorganism soybean meal leaven

Abstract

The invention relates to a preparation method of a high-efficiency compound microorganism soybean meal leaven, belonging to the technical field of biological enzymolysis and fermentation. The preparation method comprises the following steps of: respectively carrying out optimal screening on lactobacillus acidophilus, lactobacillus lactis, saccharomyces cerevisiae, Candida utilis, bacillus subtilis and bacillus licheniformis; mixing and culturing by shaking a bottle; culturing by using a 10L fermentation tank; manufacturing a microorganism compound protective agent; spray drying in low temperature; preparing the soybean meal leaven and the like. The high-efficiency compound microorganism soybean meal leaven can efficiently inhibit the growth of mixed bacterium and eliminate or remarkably lower anti-nutritional factors and flatulent factors in soybean meal, enhance the protein utilization ratio and strengthen the immunity and has the excellent characteristics of acid resistance, salt resistance, temperature resistance and bile resistance. The invention has the advantages of simple preparation method, low cost, stable and reliable produced product quality and wide application range.

Description

technical field [0001] The invention relates to a method for preparing a microbial starter, in particular to a method for preparing a high-efficiency composite microbial soybean meal starter, and belongs to the technical field of biological enzymolysis and fermentation. Background technique [0002] Soybean meal is a by-product of soybean oil extraction. Its crude protein content is as high as 40%-50%. It is rich in nutrition, low in cost, and has a reasonable composition of protein and amino acids. It is widely used as animal feed. However, soybean meal contains a large amount of anti-nutritional factors, which not only affects the utilization efficiency of protein, but also causes intestinal damage in young animals, resulting in post-weaning diarrhea in young animals, which significantly affects the health and survival of animals. The solution is to add soybean meal starter to the soybean meal to eliminate or reduce the anti-nutritional factors in the soybean meal. There ...

AI Technical Summary

Problems solved by technology

These starters have uneven vigor, slow growth, and are often contaminated by other microorganisms, resulting in unpredictable fermentations and even complete fermentation failures

Therefore, before fermentation, it is necessary to sterilize the soybean meal at high temperature, and then acidify the substrate with lactic acid to prevent contamination; air is usually required during fermentation, which is very costly