Method for extracting black fungus polysaccharide

Abstract

The invention provides a method for extracting black fungus polysaccharide, which comprises the following steps of: (1) taking dry black fungi, mechanically pulverizing the dry black fungi coarsely, screening the pulverized black fungi with a 40-mesh sieve, then pulverizing the black fungi again by an ultrafine pulverizer, and adjusting and controlling the rotating speed of a fan of the ultrafine pulverizer to between 10,000 and 22,000 r / min to obtain ultrafine powder of the black fungi; mixing the ultrafine powder of the black fungi and water in a mass ratio of 1:20-100; extracting the mixture for 20 to 90 minutes by using 10 to 40 kHz ultrasonic waves at the temperature of between 40 and 80 DEG C; centrifugating extracting solution to obtain a supernatant fluid and a deposit; adding the supernatant fluid into a mixture of diatomite and activated carbon in a mass ratio of the diatomite to the activated carbon of 1 to 3, wherein the added amount of the mixture is 2 to 8 percent of the weight of the supernatant fluid; fully stirring the mixture, than then filtering the mixture; performing membrane filtration on the filtrate by using a 50kD polysulphone membrane; and collecting macromolecular concentrated solution which is not filtered through the membrane, and drying the macromolecular concentrated solution to obtain the black fungus polysaccharide. The prepared black fungus polysaccharide has high purity and high bioactivity.

Description

(1) Technical field [0001] The invention relates to a method for extracting black fungus polysaccharide. (2) Background technology [0002] Black fungus Auricularia auricula (L.exHook.) Underwood, also known as light fungus, fine fungus, cloud ear, is a large fungus, belonging to Basidiomycetes, Auricularia, Agaricaceae, Auricularia. The polysaccharides extracted from the fruiting body of black fungus are composed of fucose, mannose, glucose and glucuronic acid, including β-(1→3), β-(1→6) and α-(1→3) Several types of glycosidic bonds, molecular weight in 1×10 5 Da~2×10 5 Da between. A large number of medical and pharmacological studies have shown that black fungus polysaccharides have anti-platelet aggregation, anti-tumor, anti-aging, antibacterial, hypoglycemic, immune function regulation, anti-ulcer, anti-hepatitis and anti-mutation, hypolipidemic and promote serum protein biosynthesis and other effects. Such as Li Wenning (2003) in the patent "preparation method of b...

AI Technical Summary

Problems solved by technology

[0006] The traditional black fungus polysaccharide extraction uses water extraction and alcohol precipitation, and the polysaccharide is difficult to diffuse out of the cell; while the acid and alkali methods easily destroy the structure of the polysaccharide and reduce its bioavailability

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