Lead ion colorimetric detection probes and application method thereof

Abstract

The invention belongs to the technical field of detection and particularly relates to lead ion colorimetric detection probes and an application method thereof. In the method, glutathione modified gold nanoparticles are used as colorimetric probes, glutathione combines with gold nanoparticles by utilizing sulfydryl groups of the glutathione for coordination to form a stable structure of which the surface contains two free carboxyl radicals. In the process of detection, the carboxyl radicals can coordinate with heavy metal ions; and by utilizing the property of surface plasma resonance of the gold nanoparticles, the existence of the heavy metal ion Pb2+ in aqueous solution can be indicated by the color change of the solution. The lead ion colorimetric detection probes have the advantages that: the sensitivity and selectivity are high; in-situ quick detection can be realized without large-size instruments; the detection results are visual and can be observed by naked eyes; the operation is simple and the cost is low; and the used solvents and the operation processes have no toxic or side effects.

Description

technical field [0001] The invention belongs to the technical field of detection, and in particular relates to a lead ion colorimetric detection probe and an application method thereof. Background technique [0002] Heavy metal ions (mercury Hg 2+ ; lead Pb 2+ ; Cadmium Cd 2+ ; Copper Cu 2+ ), especially mercury, cadmium, lead, copper, etc. have significant biological toxicity. They cannot be degraded by microorganisms in the water body, but can only undergo the process of mutual transformation, dispersion and enrichment of various forms (ie migration). Heavy metal pollution refers to environmental pollution caused by heavy metals or their compounds. It is mainly caused by human factors such as mining, exhaust gas discharge, sewage irrigation and use of heavy metal products. The degree of harm depends on the concentration and chemical form of heavy metals in the environment, food and organisms. [0003] Heavy metals generally exist in nature in natural concentrations,...

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Problems solved by technology

In recent years, nucleic acid aptamers (adapters) or DNA enzymes have been used as functional reagents to prepare probes with gold nanoparticles for the detection of Pb 2+ and other heavy metal ions [Wang Z D, Lee J H, Lu Y, Adv. Mater.2008, 20, 3263-3267; Liu J W, Lu Y, 2004, J. Am. Chem. Soc. , J W, Lu Y, 2004, Anal.Chem.76, 1627-1632; Liu J W, Lu Y, J.Am.Chem.Soc.2005, 127, 12677-12683; Wei H, Li B L, Dong S J, Wang E K, 2008, 19, 095501.], can achieve good sensitivity and selectivity, but due to the high cost of DNA and enzymes, it cannot be the choice for general application

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