Method for extracting triterpenes from schisandra chinansis

A technology of Schisandra chinensis and triterpenoids, which is applied in the field of extracting triterpenes from Schisandra chinensis, can solve the problems of high heating temperature of materials, loss of triterpenoids, complicated operation process, etc., and achieves improved extraction rate and protective activity , low-cost effect

Active Publication Date: 2010-05-19
SANMARK CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on Schisandra chinensis at home and abroad is mostly concentrated on southern Schisandra chinensis, and the research on functional components is concentrated on lignans and polysaccharides. However, there are few reports on the extraction of triterpenoids from northern Schisandra chinensis, and most reports focus on structural identification. and functional aspects
[0003] At present, the triterpenoid extraction methods of Schisandra chinensis are mostly concentrated in the reflux extraction method of ethanol or acetone solution. The operation process of this method is complicated, the heating temperature of the material is high, the loss of triterpenoids is serious, and the extraction rate is low.
At present, the separation and purification of triterpenoids usually use column chromatography, and the adsorption of the sample on the stationary phase leads to a very low recovery rate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Schisandra chinensis cane 1000g, first cut the raw material into 1cm length and then put it into a high-speed tissue homogenizer and grinder, keep it for 15min under the condition of 12000rpm, according to the ratio of liquid to material [volume of solvent (mL) / weight of raw material (g)] 13mL: After adding 1 g of 13000 mL of 80% chloroform solution, 5 g of cellulase (enzyme activity of 40000 U / g, food grade) with a weight ratio of 0.5% of the raw material was added, and after 20 min of enzymolysis at 600 W of ultrasonic power and 40 ° C, the Dry at a constant temperature of 75°C for 18 hours, crush and pass through a 60-mesh sieve as powder for future use.

[0013] Take 200g of the sieved powder and add it to the supercritical extraction kettle, set the extraction temperature to 46°C, and the temperature of the separator to 55°C to start extraction and pressurization, and adjust the CO 2 After the flow rate is constant at 18L / h and the pressure of the extraction tank i...

Embodiment 2

[0016] Schisandra chinensis cane 1000g, first cut the raw material into 1.5cm length, put it into a high-speed tissue homogenizer and grinder, keep it for 15min under the condition of 12000rpm, according to the liquid-solid ratio [solvent volume (mL) / raw material weight (g)] 13mL : After adding 13000mL of 80% chloroform solution to 1g, then add Schisandra chinensis cane raw material weight ratio of 0.5% cellulase 5g, under the condition of ultrasonic power 600W, 40°C, after enzymolysis for 20min, then dry at 75°C for 18h , crushed through a 60-mesh sieve and used as powder for later use.

[0017] Take 250g of the sieved powder and add it to the supercritical extraction kettle, set the extraction temperature to 46°C, and the temperature of the separator to 55°C to start extraction and pressurization, and adjust the CO 2 After the flow rate is constant at 18L / h and the pressure of the extraction tank is constant at 18MPa, the entrainer pump is started, and the liquid-to-materi...

Embodiment 3

[0020] Schisandra chinensis cane 1000g, first cut the raw material into 2cm length and put it into a high-speed tissue homogenizer and grinder, keep it for 15min under the condition of 12000rpm, according to the ratio of liquid to material [volume of solvent (mL) / weight of raw material (g)] 13mL: After 1 g of 80% chloroform solution 13000mL was added, then 5 g of cellulase with a weight ratio of 0.5% of Schisandra chinensis cane raw material was added. Under the condition of ultrasonic power of 600W and 40°C, after enzymatic hydrolysis for 20min, it was then dried at a constant temperature of 75°C for 18h. Crushed through a 60-mesh sieve as a powder for later use.

[0021] Take 300g of the sieved powder and add it to the supercritical extraction kettle, set the extraction temperature to 46°C, and the separation temperature to 55°C to start extraction and pressurization, and adjust the CO 2 After the flow rate is constant at 18L / h and the pressure of the extraction tank is cons...

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PUM

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Abstract

The invention relates to a method for extracting triterpenes from schisandra chinensis. The method comprises the steps of taking stems of the schisandra chinensis as main raw materials, firstly carrying out tissue breaking and cellulase and ultrasonic extraction for separating substances to be extracted from the raw materials, and then separating and extracting the triterpenes from the schisandra chinensis by utilizing the supercritical fluid extraction technology, selecting different composite entrainers and operation technological parameters and combining the high-speed countercurrent chromatography technology, thereby providing the effective method for separating and purifying the triterpenes from the schisandra chinensis. The method for extracting the triterpenes from the schisandra chinensis has the advantages of high extraction rate, simple operation process, low energy consumption, no pollution and high recovery rate, and can avoid the thermal pyrolysis of extracts at high temperature, protect the activity of physiological active substances and the like.

Description

technical field [0001] The invention relates to a method for extracting triterpenoids from Schisandra chinensis, in particular to a method for pretreatment of materials by tissue crushing, cellulase and ultrasonic extraction, and separation by supercritical fluid extraction technology combined with high-speed countercurrent chromatography technology The extraction of triterpenoids from Schisandra chinensis provides an effective method for the separation and purification of triterpenoids from Schisandra chinensis. Background technique [0002] The genus Schisandra belongs to the family Schisandra, and there are about 27 species, which are distributed in all provinces and regions in my country except Xinjiang, Qinghai, and Hainan. Among them, about 19 species are available for medicinal use, and the most medicinal value is Schisandra (commonly known as "Northern Schisandra") , followed by Huazhong Schisandra (commonly known as "Southern Schisandra"). Among them, the medicinal ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/57A61P31/12A61P35/00A61P37/04A61K135/00
Inventor 李斌孟宪军张琦
Owner SANMARK CORP
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