Method and device for preparing lipid bilayer membrane in situ in liquid phase

Abstract

The invention belongs to the technical field of membrane preparation and particularly relates to a method and a device for preparing a lipid bilayer membrane in situ in a liquid phase. Different from the common vesicle fusion method, the in-situ high-temperature vesicle fusion method adopted by the invention is characterized in that: 1, a sample introduction device is designed by combining an atomic force microscope (AFM) liquid tank needle holder to prepare a bilayer membrane in situ on a sample table; and 2, the fusion process is implemented at the temperature higher than a room temperature, so the process is accelerated. The formed bilayer membrane can be used for the observation of and study on an atomic force microscope (AFM). The method not only enables the forming process and surface topography of the membrane to be monitored by the atomic force microscope in real time, but also can change the liquid-phase environment of the membrane in situ by controlling the sample introduction device so as to control chemical and physical adsorption, chemical reactions, crystal growth and the like on the surface of the membrane. The method also can be used for preparing and modifying other amphiphilic membranes.

Description

technical field [0001] The invention belongs to the technical field of membrane preparation, and in particular relates to a preparation method and device of a lipid bilayer membrane supported by a solid surface. Background technique [0002] The planar lipid bilayer (Supported Lipid Bilayer, SLB) supported by a solid surface is one of the main forms of model biological membranes. [1，2] By controlling the composition ratio of the lipid bilayer and the properties of the medium around the membrane and other influencing factors, people can use atomic force microscopy (AFM) to qualitatively and quantitatively study the response of biological membranes to external environmental changes and the internal mechanism of specific physiological processes. [2，3] The commonly used method of preparing planar support film is spin coating method. [3] , LB membrane transfer method [3，4] and vesicle fusion [1，3] Three categories. The spin coating method is to directly spin coat the organic ...

AI Technical Summary

Problems solved by technology

However, there are some shortcomings in the various vesicle fusion methods proposed at present: (1) the time required for the spontaneous fusion of vesicles to form a membrane on a solid surface is very long, about 4 to 12 hours [1，3] (2) the probability of obtaining a better bilayer membrane is not high enough; (3) the membrane is often damaged in the process of flushing the excess vesicles on the surface; (4) in the specific implementation process, especially in the When transferred to the AFM sample stage for observation, the surface of the membrane should often contact air and lose fluidity

In addition, there are two common problems in various preparation methods at present: it is difficult to maintain the required temperature during operation and transfer, especially for multi-component systems, which may lead to irreversible phase separation and phase transition traces; it is impossible to achieve Any further modification or modification of the membrane surface so that it cannot be used for in situ modification and corresponding kinetic studies

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