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Test strip for testing psychrophiles in chilled foods and preparation method thereof

A technology of psychrophilic bacteria and test strips, which is applied in the product field of biosensing technology, can solve the problems of being unsuitable for rapid on-site detection of psychrophilic bacteria, complicated processing process, etc. , the effect of the result image

Inactive Publication Date: 2010-04-21
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The key to this patent is to use real-time fluorescence quantitative PCR technology to detect common bacteria. The sample pretreatment process of this method is complicated and requires professionals to operate, which is not suitable for rapid on-site detection of psychrophilic bacteria.

Method used

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  • Test strip for testing psychrophiles in chilled foods and preparation method thereof
  • Test strip for testing psychrophiles in chilled foods and preparation method thereof
  • Test strip for testing psychrophiles in chilled foods and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Such as figure 1 As shown, the test strips prepared in this embodiment for testing psychrophilic bacteria in refrigerated foods include: bottom plate 1, transparent membrane 2, reaction membrane 3, glass fiber sheet 4 and polyester plate 5, wherein: The glass fiber sheet 4, the reaction diaphragm 3 and the transparent diaphragm 2 are fixedly arranged between the polyester board 5 and the bottom board 1 from top to bottom in sequence, and the two ends of the glass fiber sheet 4 are connected to the inner sides of the two polyester boards 5 respectively. connection, the outer side of the polyester board 5 is fixedly connected to the base plate 1, and the center of the base plate 1 is provided with a through hole 6 facing the transparent membrane

[0026] The bottom plate 1 is made of PVC material, and the color is required to be white, which can improve the contrast value of color change and make the detection result more accurate.

[0027] The transparent membrane 2 is ...

Embodiment 2

[0037] Base plate 1, transparent film 2, glass fiber sheet 4 and polyester plate 5 of test strip, materials used are the same as embodiment 1, and concrete manufacturing process is as follows:

[0038] After laminating the polyester board cover sheet 5 and the base plate 1, punch holes with a punching machine with a hole diameter of 3-4mm.

[0039] Preparation of the reaction film layer: use 35 mg of o-benzidine hydrochloride instead. Other reagent consumption and preparation method are with embodiment 1. A small piece cut mechanically to a length of 1-2 cm covers the through hole 6 of the bottom plate 1 .

[0040] The glass fiber sheet 4 is cut into small pieces with the same size as the reaction membrane layer, and covered on the reaction membrane layer.

[0041] Fix the reaction substrate film layer and the corresponding holes of the glass fiber sheet 4 on the base plate 1 in sequence. After fixing, overlap and fix the polyester plate cover sheet 5 with the base plate 1, ...

Embodiment 3

[0044] Base plate 1, transparent film 2, glass fiber sheet 4 and polyester plate 5 of test strip, materials used are the same as embodiment 1, and concrete manufacturing process is as follows:

[0045] After laminating the polyester board cover sheet 5 and the base plate 1, punch holes with a punching machine with a hole diameter of 3-4 mm.

[0046] Preparation of the reaction film layer: 25 mg o-benzidine hydrochloride was still used, and the horseradish peroxidase was 2 mg / mL. Other reagent consumption and preparation method are with embodiment 1. A small piece cut mechanically to a length of 1-2 cm covers the through hole 6 of the bottom plate 1 .

[0047] The glass fiber sheet 4 is cut into small pieces with the same size as the reaction membrane layer, and covered on the reaction membrane layer.

[0048] Fix the reaction substrate film layer and the corresponding holes of the glass fiber sheet 4 on the base plate 1 in sequence. After fixing, overlap and fix the polyeste...

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Abstract

The invention discloses a test strip for testing psychrophiles in chilled foods and a preparation method thereof, which belong to the technical field of biosensors. The test strip comprises a substrate, a transparent membrane, a reaction membrane, a glass fiber piece and polyester plates, wherein the glass fiber piece, the reaction membrane and the transparent membrane are sequentially and fixedly arranged between the polyester plates and the substrate from top down; the two ends of the glass fiber piece are connected with the inner sides of two polyester plates respectively; the outer sides of the polyester plates are fixedly connected with the substrate; and the center of the substrate is provided with a through hole just opposite to the transparent membrane. The test strip has high sensitivity, high accuracy, good repeatability and a wide application range and can be effectively preserved for more than one year.

Description

technical field [0001] The invention relates to a product in the technical field of biosensing, in particular to a test strip for testing psychrophilic bacteria in refrigerated food and a preparation method thereof. Background technique [0002] Psychrophilic bacteria is a kind of bacteria with strong antifreeze ability. Generally, it is most suitable for growth between 0°C and 20°C. Therefore, it brings certain difficulties to low-temperature storage of food, such as meat, milk and dairy products, soy products, salads, etc. And aquatic products, etc., are more susceptible to its contamination of the food. Most psychrophilic bacteria have been generally recognized worldwide as pathogens of zoonotic and foodborne diseases in etiology. At present, the methods for detecting psychrophilic bacteria are mainly traditional bacterial identification methods and immunological detection methods such as PCR and ELISA. The traditional bacterial identification methods are cumbersome and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/28C12Q1/06G01N21/78
Inventor 柴春彦刘国艳姚冰刘晓芳王莉
Owner SHANGHAI JIAO TONG UNIV
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