Preparation method of enzyme hydrolysis pearl powder
A manufacturing method and technology of pearl powder, applied in skin care preparations, medical preparations containing active ingredients, food preparation, etc., can solve problems such as difficult absorption, and achieve easy absorption, high hydrolysis rate, and simple process methods Effect
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Embodiment 1
[0015] 10kg of pearls were pulverized by an ultrafine pulverizer to obtain 9.87kg of pearl powder. Detected with a MASTERSIZER200 laser particle size analyzer, the particle size results of the pearl powder are: d (0.1) 4.355 microns, d (0.5) 9.023 microns, d (0.9) 20.062 microns; Protein 3.2g. Gained pearl powder 9.83kg is mixed with water into 20% turbid liquid.
[0016] Pour the turbid solution into a 100L bioreactor, heat to 45°C, add 8 grams of neutral protease, stir for 4 hours to carry out enzymatic hydrolysis, and then raise the temperature to 100°C to inactivate the enzyme.
[0017] The turbid liquid after hydrolysis was dried with a spray dryer to obtain 9.80 kg of enzyme-hydrolyzed pearl powder.
[0018] Inspection: Take 10g of enzyme-hydrolyzed pearl powder and 100g of water, mix evenly, centrifuge at 1000 rpm, take the supernatant for total protein (amino acid, total polypeptide) detection (Kjeldahl method), get 0.06g of soluble total protein, pearl powder Conta...
Embodiment 2
[0024] 10kg of pearls were pulverized by an ultrafine pulverizer to obtain 9.90kg of pearl powder. Detect with a MASTERSIZER200 laser particle size analyzer, the pearl powder particle size results are: d (0.1) 3.955 microns, d (0.5) 8.822 microns, d (0.9) 19.06 microns, and the pearl powder contained in every 100g of pearl powder is measured by the Nitrogen method. Protein 3.13g. Gained pearl powder 9.85kg and water are mixed with 50% slurry. Then use a small ball mill to wet grind until the average particle size is less than 0.5 micron. Detected with a MASTERSIZER200 laser particle size analyzer, the pearl powder particle size results are: d (0.1) 0.172 microns, d (0.5) 0.365 microns, d (0.9) 0.898 microns.
[0025] Mix the wet-milled pearl powder with water to form a turbid liquid containing 40% pearl powder.
[0026] Pour the turbid solution into a 100L bioreactor, heat it to 50°C, add 10 grams of neutral protease, stir for 6 hours to carry out enzymatic hydrolysis, and ...
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