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Method for measuring content of phospholipid in liposome

A technology of phospholipid content and determination method, which is applied in the preparation of test samples and the measurement of color/spectral properties, etc., can solve the problems of unreasonable ratio of color reagent solution, difficult temperature control, complicated operation steps, etc., and achieve the display effect. Good, improve the accuracy, reduce the effect of environmental pollution

Inactive Publication Date: 2011-08-10
NORTH CHINA PHARMA COMPANY
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  • Abstract
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  • Claims
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Problems solved by technology

Although this method is specially designed for the detection of phospholipids in liposomes, the ratio of the chromogenic reagent solution is unreasonable, and the chromogenic results are poor; and there are many kinds of reagents and complicated operation steps.
In the detection process, electric furnace heating is required, the temperature is not easy to control, and it is easy to cause bumping phenomenon

Method used

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  • Method for measuring content of phospholipid in liposome
  • Method for measuring content of phospholipid in liposome
  • Method for measuring content of phospholipid in liposome

Examples

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Effect test

Embodiment

[0049] Adopt method of the present invention to carry out liposome content determination to commercially available amphotericin B liposome for injection

[0050] (1) Tested sample:

[0051] Amphotericin B liposome for injection (Amphotec) 50mg: 12ml

[0052] Manufacturer: Ben Venue Laboratorics.Inc.USA

[0053]Production batch number: 141087E, 141088E, 141089E

[0054] (2) Determination method:

[0055] a. Preparation of chromogenic agent

[0056] A Chromogenic Agent: Accurately weigh 0.22g of ammonium molybdate, dissolve it in distilled water and pour it into a 100ml volumetric flask, then slowly add 4.72ml of analytically pure sulfuric acid, let it cool, dilute to the mark, and pour it into a brown bottle Store in the refrigerator;

[0057] B Chromogen: Accurately weigh 15.00 g of L-ascorbic acid in a beaker, and use 14% Na 2 SO 3 Dissolve and dilute to a 100ml volumetric flask, pour into a brown bottle and store in the refrigerator;

[0058] b. Preparation of phosph...

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Abstract

The invention discloses a method for measuring the content of phospholipid in liposome, which comprises the following steps: preparing a color development reagent A and a color development reagent B; preparing standard storage liquid; preparing a sample solution; drawing a standard curve; measuring the content of a sample: accurately sucking 0.5ml of the prepared liposome sample solution into 15*200mm test tubes, and sequentially adding 0.4ml of sulfuric acid with the concentration of 10N and 0.1ml of 30% hydrogen peroxide solution; heating for 60 minutes at a constant temperature of 170 DEG C; after cooling, adding 9ml of distilled water into each test tube, uniformly mixing and sucking 1.5ml of mixed solution into another test tube, then respectively adding 4.6ml of solution of the color development reagent A and 0.1ml of solution of the color development reagent B, and heating for 15 minutes in a water bath at 100 DEG C; after cooling, carrying out color comparison at the point with the wavelength of 822nm to measure the absorbance; and substituting the obtained absorbance value of the sample into a regression curve equation to calculate the content of phosphorus in the sample.The invention has simple and convenient method and easy operation, greatly improves the accuracy of the measuring result, and also improves the precision of the measuring result.

Description

technical field [0001] The invention relates to a method for quality control of a drug carrier, in particular to a method for determining the content of phospholipids in liposomes. Background technique [0002] Liposomes are lipid microcapsules with an aqueous inner core composed of phospholipid bilayers. Due to its characteristics of slow release, low toxicity and tissue targeting, it has been used in many aspects such as drug delivery and gene transfer. Phospholipids are one of the important components of liposomes. It is prone to hydrolysis and oxidation, hydrolysis will lead to leakage of encapsulated drugs in liposomes, and the oxidation reaction produced by oxidation has certain toxicity to the human body. Therefore, the determination of phospholipid content is an important index to measure the stability of liposomes. At present, the methods for determining the content of phospholipids mainly include high-performance liquid chromatography, molybdenum blue colorimetr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/31G01N1/28
Inventor 王永维刘浩陈剑琴张莉张永祥刘英华韩新峰
Owner NORTH CHINA PHARMA COMPANY
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