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Candida bacterial strain and method for producing D-arabitol

A technology of Candida and arabinol, applied in microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve problems such as limiting the industrial application of biological methods

Active Publication Date: 2010-04-07
HANGZHOU BIOKING BIOCHEM ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, the high-yielding D-arabinol strains reported in Table 1 are mainly fungi, and the conversion rate of D-arabinol to glucose in most strains is about 30% to 40%, and the fermentation period is 72 hours to 300 hours. , Glycerin, other sugar alcohols and other by-products, these factors directly limit the industrial application of biological methods

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Take a small amount of samples such as fresh fruit, rotten fruit, pollen, soil, honeycomb and fresh honey, and cultivate them in a solid medium for 3 days; then use the coating method to spread on a preserved solid medium for 2-3 days; select different colony forms The strains were stored in solid medium and stored in a refrigerator at 4°C until use. A total of 420 strains of hypertonic strains were screened in this embodiment.

[0032] The hyperosmotic strain was inoculated and cultured in the fermentation broth for 6 days; the fermentation broth was centrifuged to leave the supernatant; and the D-arabinol was qualitatively detected by thin-layer chromatography. The development system of the thin-layer chromatography method involved in the present invention is butanone: acetone: water=10: 1: 1 (ml / ml / ml), and the color development method is sodium periodate-benzidine method (1g / L high Sodium iodate; 1.4g benzidine dissolved in 80ml 95% ethanol, 70ml distilled water, 3...

Embodiment 2

[0057] The slant of the above-mentioned Candida sp.H2 cultured for 2 to 3 days (glucose 100g / l, yeast extract 10g / l, agar powder 15g / l, sterilized at 115°C for 20 minutes) was placed in a container In a 100ml Erlenmeyer flask with 20ml of seed liquid medium (glucose 20g / l, yeast extract 10g / l, sterilized at 121°C for 20 minutes), shake culture at 30°C and 200rpm for 24 hours, absorb 1ml of the above-mentioned Candida H2 The seed bacteria liquid of (Candidasp.H2) was placed in a 500ml Erlenmeyer flask containing 100ml fermentation liquid medium (glucose 100g / l, yeast extract 10g / l, pH 7.0, sterilized at 115°C for 20 minutes), at 30°C , 200rpm shaking culture for 144 hours, the results are shown in Table 3.

[0058] table 3

[0059]

Embodiment 3～ Embodiment 8

[0061] In the culture methods of Examples 3 to 8, the concentration of glucose relative to the fermentation liquid medium is shown in Table 1, and the rest of the culture conditions are the same as in Example 2, and the results are shown in Table 3.

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Abstract

The invention discloses a Candida bacterial strain and a method for producing D-arabitol. The Candida sp.H2 has the preservation number of CGMCC No.3255 in China Committee of Culture Collection for Microorganisms (CCCCM), and the 26S rDNA D1 / D2 region sequence of the bacterial strain is displayed in SEQ IC NO:1. In the invention, the method using the Candida bacterial strain to produce D-arabitol is as follow: cultivating the Candida bacterial strain in a seed liquid culture medium containing glucose and yeast extracts to obtain the inoculum of Candida; then inoculating the inoculum of Candida to the seed liquid culture medium containing glucose and yeast extracts to ferment and produce D-arabitol. Compared with the prior art, the invention provides a new Candida bacterial strain which can be used for producing D-arabitol. The D-arabitol produced with the Candida sp.H2 of the invention has higher yield, and the accumulation amount can reach the high level of 86.55g.

Description

technical field [0001] The invention relates to a Candida strain and a method for using the Candida to metabolize glucose into D-arabinol. Background technique [0002] D-arabitol (D-arabitol) is a pentasaccharide alcohol that widely exists in nature and is one of the important products of hypertonic microorganisms. It is mainly used in food, chemical, pharmaceutical and other industries. At present, the most potential market use of D-arabinol is as a raw material for the synthesis of xylitol. [0003] The research on strains accumulating D-arabinol began in the middle of the 20th century abroad, but it started relatively late in China and so far there are few reports. According to reports, the vast majority of hyperosmotic-resistant strains can synthesize D-arabinol, the possible reason is that D-arabinol can protect cells under hyperosmotic conditions, mainly including Aspergillus (Aspergillus), Candida ( Candida, Debaryomyces, Moniliella, Hansenula, Kodamae, Metschnikow...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12P7/18C12R1/72
Inventor 张建国谢志鹏宋卫斌
Owner HANGZHOU BIOKING BIOCHEM ENG
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