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Propolis-astragalus polysaccharides compound nanometer emulsion adjuvant and preparation method and application thereof

A technology of astragalus polysaccharide and nanoemulsion, which is applied in antiviral agents, pharmaceutical formulations, emulsion delivery, etc., to achieve the effects of low viscosity, enhanced immune function, and excellent stability

Inactive Publication Date: 2010-04-07
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the fatal disadvantage of inactivated vaccines and genetically engineered vaccines is that they cannot induce T cell immune responses, but in inducing more effective antiviral immune responses, the participation of T cells is essential, and adjuvants can assist antigens to induce T cells. cellular immunity

Method used

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  • Propolis-astragalus polysaccharides compound nanometer emulsion adjuvant and preparation method and application thereof
  • Propolis-astragalus polysaccharides compound nanometer emulsion adjuvant and preparation method and application thereof
  • Propolis-astragalus polysaccharides compound nanometer emulsion adjuvant and preparation method and application thereof

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Experimental program
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Embodiment 1

[0031] The preparation of embodiment 1 propolis and astragalus polysaccharide compound nanoemulsion adjuvant

[0032] Prepare the recipe as follows:

[0033] Propolis 1.0g

[0034] RH-40 33.0g

[0035] Absolute ethanol 16.5g

[0036] Ethyl acetate 5.5g

[0037] Astragalus Polysaccharide 1.0g

[0038] Distilled water 42.5g

[0039] The specific operation steps are:

[0040] 1) Weigh propolis, astragalus polysaccharide, surfactant, co-surfactant, oil and distilled water respectively according to the prescription ratio;

[0041] 2) Completely dissolve the propolis of the prescribed amount in the co-surfactant (the co-surfactant can be used as a solvent here), then add the oil phase, finally add the surfactant and mix well, set aside.

[0042] 3) Dissolving astragalus polysaccharide in distilled water according to the prescription amount;

[0043] 4) Slowly add the above distilled water into the system dropwise at room temperature, while stirring continuously, until a unif...

Embodiment 2

[0044] Embodiment 2 propolis and astragalus polysaccharide compound nanoemulsion adjuvant

[0045] Propolis 0.01g

[0046] RH-40 33.0g

[0047] Absolute ethanol 16.5g

[0048] Ethyl acetate 5.5g

[0049] Astragalus polysaccharide 0.1g

[0050] Distilled water 37.5g

Embodiment 3

[0051] Example 3 Propolis and astragalus polysaccharide compound nanoemulsion adjuvant

[0052] Propolis 0.1g

[0053]RH-40 33.0g

[0054] Absolute ethanol 16.5g

[0055] Ethyl acetate 5.5g

[0056] Astragalus Polysaccharide 14.0g

[0057] Distilled water 34.5g

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Abstract

The invention discloses a propolis-astragalus polysaccharides compound nanometer emulsion adjuvant which comprises the following components by weight percent: 0.01%-6% of propolis, 0.01%-14% of astragalus polysaccharides, 16.6%-54.0% of surfactant, 6.3%-30.5% of cosurfactant, 5.1%-25.2% of oil and 34.0%-60.0% of distilled water. The sum of the weight percentages of the components is 100%. The compound nanometer emulsion adjuvant is brown-yellow transparent oil-in-water nanometer emulsion with the grain size of 1-100nm. The compound nanometer emulsion adjuvant can be directly used to prepare vaccine along with various inactivated antigens and the mass production can be realized without any special emulsifying device. Meanwhile, the compound adjuvant has the advantage of good stability, high safety, low viscosity and the like.

Description

technical field [0001] The invention relates to a novel vaccine adjuvant, in particular to a preparation method of a compound nanoemulsion adjuvant of propolis and astragalus polysaccharide and its application in vaccine production. technical background [0002] Because live vaccines have many safety problems, inactivated vaccines and various new vaccines have attracted more and more attention. However, inactivated vaccines and various new vaccines generally have the characteristics of poor immunogenicity, which cannot play a good clinical protective effect. In order to enhance the immune effect of the vaccine, it is necessary to add an adjuvant to enhance its immunogenicity. Therefore, the development of a safe and stable immune adjuvant has become a focus of attention in recent years. At present, the fatal disadvantage of inactivated vaccines and genetically engineered vaccines is that they cannot induce T cell immune responses, but in inducing more effective antiviral im...

Claims

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Application Information

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IPC IPC(8): A61K39/39A61K39/145A61K39/17A61K39/12A61K9/107A61K47/44A61P31/14A61P31/16
Inventor 欧阳五庆张旭李雅
Owner NORTHWEST A & F UNIV
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