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Particle blood platelet dry powder preparation and preparation method thereof and application thereof

A technology of dry powder preparation and platelet activator, which is applied in the field of biopharmaceuticals to achieve the effect of promoting wound healing, saving blood resources, and good wound healing.

Inactive Publication Date: 2011-07-27
THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the application of platelets requires ABO blood type compatibility, but the platelet particles made by mixing platelets from multiple people can destroy or mask blood group antigens, regardless of the recipient's ABO blood type

Method used

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  • Particle blood platelet dry powder preparation and preparation method thereof and application thereof
  • Particle blood platelet dry powder preparation and preparation method thereof and application thereof
  • Particle blood platelet dry powder preparation and preparation method thereof and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1, the preparation of particulate platelet dry powder preparation

[0020] Take 3ml of expired apheresis platelets from 5 servings, mix them, and dilute them with platelet preservation solution to a concentration of 1.5×10 9 / ml, take 20ml of the diluted platelets, add 1ml of plasma with albumin concentration ≥ 45g / L and 1ml of adenosine diphosphate solution with a concentration of 40μmol / L, mix well, and act for 0.5 hours at room temperature to obtain activated platelets; Freeze at -80°C for 24 hours, then thaw at room temperature for 1 hour, freeze and thaw repeatedly 3 times to lyse the platelets to obtain platelet particles; heat the platelet particles at 60°C for 10 hours to inactivate the virus, add a mass fraction of 20 % starch slurry, mixed evenly, and freeze-dried for 24 hours to obtain a microparticle-type platelet dry powder preparation.

Embodiment 2

[0021] Embodiment 2, the preparation of particulate platelet dry powder preparation

[0022] Take 3ml of expired apheresis platelets from 5 servings, mix them, and dilute them with platelet preservation solution to a concentration of 2×10 9 / ml, take 5ml of the diluted platelets, add 1ml of plasma with albumin concentration ≥ 45g / L and 1ml of adenosine diphosphate solution with a concentration of 40μmol / L, mix well, and act for 0.5 hours at room temperature to obtain activated platelets; Freeze at -80°C for 24 hours, then thaw at room temperature for 1 hour, freeze and thaw repeatedly 3 times to lyse platelets to obtain platelet particles; heat the obtained platelet particles at 60°C for 10 hours to inactivate the virus, add mass fraction of 0.1 ml of 30% starch slurry was mixed evenly, and freeze-dried for 24 hours to obtain a microparticle-type platelet dry powder preparation.

Embodiment 3

[0023] Embodiment 3, the preparation of particulate platelet dry powder preparation

[0024] Take 3ml of expired apheresis platelets from 5 servings, mix them, and dilute them with platelet preservation solution to a concentration of 1×10 9 / ml, take 30ml of the diluted platelets, add 1ml of plasma with albumin concentration ≥ 45g / L and 1ml of adenosine diphosphate solution with a concentration of 40μmol / L, mix well, and act for 2 hours at room temperature to obtain activated platelets; Freeze at -80°C for 24 hours, then thaw at room temperature for 1 hour, freeze and thaw repeatedly 3 times to lyse the platelets to obtain platelet particles; heat the platelet particles at 60°C for 10 hours to inactivate the virus, add a mass fraction of 10 % starch 20ml, mixed evenly, and freeze-dried for 24 hours to obtain the microparticle platelet dry powder preparation.

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Abstract

The invention discloses a particle blood platelet dry powder preparation and a preparation method thereof and the application thereof; the preparation is formed by the following constituents by volume parts: 5-30 parts of blood platelet with 1*10<9>-2*10<9> of concentration, 0.1-10 parts of blood plasma with more than or equal to 45g / L of albumin, and 0.1-20 parts of 10-30 percent of starch slurry by mass percentage; the preparation method is that: the blood plasma and blood platelet activators are added in blood platelet of single person or mixed blood platelet of a plurality of persons, andthe reaction is carried out for 0.5-2 hours at room temperature, so as to obtain activated blood platelet; freeze thawing is carried out to the activated blood platelet repeatedly or high-speed homogenization is carried out to the activated blood platelet to lead the blood platelet to be cracked into blood platelet particles; after the blood platelet particles carry out virus inactivation, starchslurry is added, the mixture is mixed uniformly and then is frozen and dried to obtain the preparation; the blood platelet in the invention can be overdue blood platelet in clinical application, and the preparation can be stored for a long time, thereby having good effect on promoting wound healing and being capable of being used for prepare medicines for promoting wound healing.

Description

technical field [0001] The invention relates to a microparticle platelet dry powder preparation, a preparation method and an application of the preparation, and belongs to the field of biopharmaceuticals. Background technique [0002] Wound healing is an extremely complex process that involves many pathophysiological changes in the body, such as coagulation, inflammation, proliferation, regeneration, and reorganization. Many studies have shown that: growth factors [such as platelet-derived growth factor (PDGF), vascular endothelial cell growth factor (VEGF), basic fibroblast growth factor (bFGF), epidermal growth factor (EGF), insulin-like growth factor (IGF ), transforming growth factor beta 1 (TGF-β 1 ) etc.] can promote wound healing, and the combined application of multiple growth factors is better than a single factor. Other studies have shown that concentrated platelets have unique advantages for wound repair, which may be due to the release of various growth factor...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/14A61K35/16A61K47/26A61P17/02
Inventor 赵树铭黎儒青李静范娅涵蒋天伦
Owner THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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