Human Bcl-2 and human VEGF165 double-gene co-expression recombinant vector and building method thereof
A technology for recombinant vectors and construction methods, which is applied in botany equipment and methods, biochemical equipment and methods, genetic engineering, etc., can solve unsatisfactory problems, achieve the effects of accelerating recovery, promoting angiogenesis, and broad application prospects
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[0041] Figure 1-11 Constitute the specific embodiment 1 of the present invention.
[0042] Human Bcl-2 and Human VEGF 165 Double-gene co-expression recombinant vector, including human Bcl-2 gene and human VEGF 165 Gene; wherein, the human Bcl-2 gene sequence is described in the sequence table SEQ ID No. 1; human VEGF 165 The gene sequence is as described in SEQ ID No. 2 in the sequence table; the vector is an adenovirus vector, and other vectors such as other adeno-associated viruses, lentiviruses and plasmids can also be used.
[0043] The human Bcl-2 and human VEGF 165 The construction process of the double gene co-expression recombinant adenoviral vector is as follows: figure 1 As shown, its construction method is as follows:
[0044] 1. Main experimental reagents and equipment:
[0045] Biochemical reagents: DNA Marker (Takara, Japan), tryptone, yeast extract, agar powder (Oxoid, Japan), agarose (Biowest, Spain), kanamycin, ampicillin (Amresco, USA) and Trizol reage...
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