Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Molecular detection method for rapidly distinguishing mating types of Chinese caterpillar fungus

A molecular detection and mating type technology, applied in the biological field, can solve the problem of inability to effectively distinguish the mating type of domestic Cordyceps militaris strains, and achieve the effects of reliable detection results, improved yield and quality, and increased probability of weeding

Inactive Publication Date: 2012-08-22
CHINA AGRI UNIV
View PDF0 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in our experiments, the degenerate primers designed by Yokoyama et al. could not effectively distinguish the mating types of domestic Cordyceps militaris strains.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Molecular detection method for rapidly distinguishing mating types of Chinese caterpillar fungus
  • Molecular detection method for rapidly distinguishing mating types of Chinese caterpillar fungus
  • Molecular detection method for rapidly distinguishing mating types of Chinese caterpillar fungus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1: Primer Cm1-F / Cm1-R and Cm2-F / Cm2-R detect the mating type of Cordyceps militaris monoconidia strain

[0034] Using primers Cm1-F / Cm1-R and Cm2-F / Cm2-R, the mating type of 14 monoconidia strains of Cordyceps militaris was detected rapidly. Primers were synthesized by Shanghai Sangon Bioengineering Technology Service Co., Ltd. PCR amplification system includes 10×buffer 2.5μL, 2.5mM dNTP 2.0μL, 10mM forward and reverse primers 0.5μL each, 2.5U / μL Taq DNA polymerase 0.2μL, 10ng / μL DNA template 1.0μL, ddH 2 O to make up to 25 μL. The PCR amplification program for the primer pair Cm1-F / Cm1-R was as follows: pre-denaturation at 95°C for 5 minutes; 95°C for 40s, 60°C for 30s, and 72°C for 30s; followed by 95°C for 40s, 58°C for 30s, 72°C Amplify at ℃30s for 24 cycles; finally extend at 72℃ for 10min; store at 4℃. The PCR amplification program for the primer pair Cm2-F / Cm2-R is: pre-denaturation at 95°C for 5 minutes; 95°C for 40s, 66°C for 30s, and 72°C for 30...

Embodiment 2

[0035] Example 2: Primers Cm1-F / Cm1-R and Cm2-F / Cm2-R detect the mating type of the Cordyceps militaris monoascospore strain.

[0036] Using primers Cm1-F / Cm1-R and Cm2-F / Cm2-R to detect the mating type of 13 Cordyceps militaris monoascospores. The PCR amplification system and amplification procedure are all the same as the steps of detecting the mating type of the Cordyceps militaris monoconidia strain of Example 1. The primer pair Cm1-F / Cm1-R can specifically amplify a band of about 180bp in the monoascospore strain of MAT1-1 mating type. The detection results are shown in image 3 ; The primer pair Cm2-F / Cm2-R can be specifically amplified to obtain a band of about 210bp in the monoascospore strain of MAT1-2 mating type, and the detection results are shown in Figure 4 .

Description of drawings

[0037] image 3 Electrophoresis diagram of PCR amplification of DNA of Cordyceps militaris monoascospores by specific primers Cm1-F / Cm1-R: M stands for Marker-DL2000; lanes 1 ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a molecular detection method for rapidly distinguishing MAT1-1 and MAT1-2 mating types of Chinese caterpillar fungus, comprising two pairs of specific primers (Cm1-F / Cm1-R and Cm2-F / Cm2-R) for detecting mating types and two sets of corresponding PCR amplification systems and PCR amplification programs. The primer pair Cm1-F / Cm1-R is used for detecting the MAT1-1 mating type of Chinese caterpillar fungus, and the other primer pair Cm2-F / Cm2-R is used for detecting the MAT1-2 mating type of Chinese caterpillar fungus; and using both pairs of the specific primers and thecorresponding PCR amplification systems and the PCR amplification programs can realize the rapid detection of the mating types of Chinese caterpillar fungus. The detection results of the molecular detection method have high specificity, and the molecular detection method is stable, reliable and rapid and can greatly shorten the time of biological detection. The molecular detection method is suitable for the detection of the mating types of Chinese caterpillar fungus in the relevant professional fields such as production of edible fungi and medicinal fungi, strain breeding, plant protection and the like as well as the research in the aspects of mating type gene, phylogenetic development and the like, and has significance in researching sexual reproduction of other fungi of cordyceps and directing the production practice.

Description

technical field [0001] The invention relates to a biotechnology, in particular to a molecular detection method for quickly distinguishing the mating type of Cordyceps militaris. The technology of the invention is applicable to the detection of the mating type of Cordyceps militaris and the research on the mating type gene and phylogenetic development of edible and medicinal fungi, strain selection, plant protection and other related professional fields. Background technique [0002] The mating system of fungi is mainly divided into homothallic (self-fertile) and heterothallic (self-sterile), and heterothallic strains must be mated by individuals of different mating types to complete sexual reproduction. The mating type genes of heterothallic strains regulate the sexual compatibility between individuals and play a decisive role in sexual reproduction, fruiting body differentiation, development, and genetic evolution. Therefore, the research on the mating type of fungi in nat...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12R1/645
Inventor 张国珍袁雪芬
Owner CHINA AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com