Method for quickly extracting and preserving silica gel membrane type DNA

Abstract

The invention discloses a method for quickly extracting and preserving a silica gel membrane type DNA. By utilizing the characteristic of a silica gel membrane that the silica gel membrane adsorbs DNAunder the conditions of high salt and low pH and releases the DNA under the conditions of low salt and high pH, the invention does not need a process of extracting the DNA in advance after the silicagel membrane is processed by a novel solution containing a cell cracking agent and a nuclease suppressor, cells are directly lysed, the DNA is adsorbed on the silica gel membrane, the silica gel membrane adsorbing the DNA can be preserved for a long time because of the existence of the nuclease suppressor and the degradation of the DNA is avoided. In the extracting process, the pure DNA is adsorbed on the membrane by the elution function of eluent I after salt and cell residues on the membrane are rinsed. By the elution function of eluent II, the DNA combined on the membrane is eluted into the solution. The whole DNA extracting and preserving process has quick operation and low price and is simple and convenient.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for extracting and preserving DNA from bacteria or body fluids by using a silica gel membrane. Background technique [0002] At present, the technology of extracting DNA from bacteria or body fluids has been widely used, and there are various methods for extracting and preserving DNA. [0003] The traditional DNA extraction method is the phenol-chloroform method, which is cheap and widely used, but it is easy to cause environmental pollution due to the large amount of organic solvent used. The DNA extraction kit developed by using the DNA adsorption properties of resin, silica gel and silica gel membrane has little environmental pollution, but the operation steps are cumbersome, and the purified DNA needs to be obtained by multi-step centrifugation after the lysate is lysed. DNA cannot be preserved for long periods of time. [0004] The commonly used technology for DNA pre...

AI Technical Summary

Problems solved by technology

The disadvantage of using method 1 is that laboratory equipment such as centrifuges and ultra-clean workbenches are required at the collection site, and this method cannot be used to extract and preserve DNA for samples collected in the field or places without laboratory conditions

The disadvantage of using method 2 is that it is inconvenient to transport bacteria liquid or body fluid at low temperature. If DNA extraction is not required immediately, the sample must be stored at low temperature all the time, which is costly.

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