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Joint detection kit for semen

A combined detection and kit technology, applied in the field of technical detection, can solve the problems of complicated operation, long time, inconvenient storage and use, etc., and achieve the effect of obvious comprehensive advantages and accurate inspection basis.

Inactive Publication Date: 2019-02-26
山东康华生物医疗科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the detection of these items in clinical practice is complicated, takes a long time, and requires special equipment to operate on the computer
In addition, the existing test paper must be stored at 2-8°C, which is inconvenient to store and use

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1: A combined semen detection kit, including a sample diluent, a color developing solution and a detection card.

[0050] The sample diluent is prepared from NaCl and water, and the concentration of NaCl is 0.85%.

[0051] The chromogenic solution includes lactate dehydrogenase chromogenic solution and carnitine chromogenic solution;

[0052] The lactate dehydrogenase chromogenic solution is prepared from PBS buffer and NADH, the pH of the PBS buffer is 7.2, the concentration is 0.015M, and the concentration of NADH is 1mmol / L;

[0053] The carnitine chromogenic solution is prepared from PBS buffer and acetyl-CoA, the pH of the PBS buffer is 7.2, the concentration is 0.015M, and the concentration of acetyl-CoA is 0.1g / L.

[0054] The detection card is provided with several reaction wells, and each reaction well is equipped with pH value detection paper, leukocyte lipase detection paper, carnitine detection paper, lactate dehydrogenase detection paper, lecith...

Embodiment 2

[0088] Embodiment 2: A combined semen detection kit, including a sample diluent, a color developing solution and a detection card.

[0089] The sample diluent is prepared from NaCl and water, and the concentration of NaCl is 0.87%.

[0090] The chromogenic solution includes lactate dehydrogenase chromogenic solution and carnitine chromogenic solution;

[0091] The lactate dehydrogenase chromogenic solution is prepared from PBS buffer and NADH, the pH of the PBS buffer is 7.4, the concentration is 0.02M, and the concentration of NADH is 25mmol / L;

[0092] The carnitine chromogenic solution is prepared from PBS buffer and acetyl-CoA, the pH of the PBS buffer is 7.24, the concentration is 0.02M, and the concentration of acetyl-CoA is 50g / L.

[0093] The detection card is provided with a number of reaction holes, and each reaction hole is placed with pH value detection paper or leukocyte lipase detection paper or carnitine detection paper or lactate dehydrogenase detection paper ...

Embodiment 3

[0127] Embodiment 3: A combined semen detection kit, including a sample diluent, a color developing solution and a detection card.

[0128] The sample diluent is prepared from NaCl and water, and the concentration of NaCl is 0.9%.

[0129] The chromogenic solution includes lactate dehydrogenase chromogenic solution and carnitine chromogenic solution;

[0130] The lactate dehydrogenase chromogenic solution is prepared from PBS buffer and NADH, the pH of the PBS buffer is 7.6, the concentration is 0.025M, and the concentration of NADH is 50mmol / L;

[0131] The carnitine chromogenic solution is prepared from PBS buffer and acetyl-CoA, the pH of the PBS buffer is 7.6, the concentration is 0.025M, and the concentration of acetyl-CoA is 100g / L.

[0132] The detection card is provided with a number of reaction holes, and each reaction hole is placed with pH value detection paper or leukocyte lipase detection paper or carnitine detection paper or lactate dehydrogenase detection paper...

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Abstract

The invention provides a joint detection kit for semen. The joint detection kit is characterized by comprising a sample diluent, a developing solution, pH value detection paper and / or leukocyte lipasedetection paper and / or carnitine detection paper and / or lactic dehydrogenase detection paper and / or lecithin body detection paper and / or citric acid detection paper and / or zinc detection paper and / orelastase detection paper and / or fructose detection paper and / or neutral-alpha-glucosidase detection paper. Due to the adoption of technical scheme, compared with the prior art, the joint detection kit provided by the invention has the advantages that computer practice can be realized; the joint detection kit can replace a traditional detection method and has obvious comprehensive advantages in aspects of sensitivity, accuracy, preservation method, usable range, cost performance, operation and the like; after the detection paper is soaked into stabilizing fluid and then is dried, long-term normal temperature preservation can be realized; the joint detection kit provides scientific and accurate inspection standards for microecological environment assessment of seminal plasma and diagnosis of various male infertilities.

Description

technical field [0001] The invention relates to the field of technical detection. [0002] Specifically, it relates to a semen combined detection kit. Background technique [0003] The incidence of male infertility is about 10%. Among them, the purely female factor is about 50%, the purely male factor is about 30%, and the total of men and women is about 20%. Clinically, male infertility is divided into sexual dysfunction and normal sexual function. The latter can be further divided into azoospermia, oligospermia, asthenozoospermia, spermatozoa and normal sperm count infertility according to the results of semen analysis. . [0004] The causes of male infertility are more complicated. The main reasons are abnormal semen, spermatogenesis disorder, sperm-egg combination disorder and other systemic factors, such as mental and environmental factors, nutritional factors and endocrine diseases. Abnormal semen is the main cause of male infertility. [0005] Normal semen is one...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50G01N33/52
CPCG01N33/5005G01N33/52G01N2333/46
Inventor 杨致亭魏红李俊美邱香廷潘德芹
Owner 山东康华生物医疗科技股份有限公司
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