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Application of osthole in preparing anti-angiogenic drugs

A technology of angiogenesis and osthole, which is applied in drug combinations, antipyretics, antineoplastics, etc.

Inactive Publication Date: 2010-02-17
EAST CHINA NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, there is no report that osthole can inhibit angiogenesis or inhibit tumors through angiogenesis.

Method used

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  • Application of osthole in preparing anti-angiogenic drugs
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  • Application of osthole in preparing anti-angiogenic drugs

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0020] Experimental example 1: In vitro proliferation experiment of osthole inhibiting human umbilical vein endothelial cells

[0021] Purpose and principle: MTS assay was used in endothelial cell proliferation assay. The MTS assay is an assay that uses a colorimetric method to indirectly measure the number of viable cells. In living cells, NADPH dehydrogenase in mitochondria converts MTS tetrazolium salt compound into a colored substance - formazan, which is soluble in cell culture fluid, and its color depth is highly correlated with the number of living cells of the cell line within a certain range. The effect of drugs on cell proliferation can be evaluated by this method.

[0022] Method: Inoculate human umbilical vein endothelial cells into 96-well plates (5000 cells / well), add medium containing different concentrations of osthole for 48-72 hours, then add chromogenic reagent and incubate for 1-4 hours. Read the absorbance of each well at 490 nm with a microplate reader....

Embodiment 2

[0024] Embodiment 2: Osthole inhibits human umbilical vein endothelial cell migration experiment in vitro

[0025] Purpose and principle: Cell migration test is also called in vitro scratch test. In this experiment, scratches were made on the overgrown monolayer endothelial cells in the culture plate, observed under a microscope, and the number of newly immigrated endothelial cells at the scratches was recorded to evaluate the effect of drugs on the migration ability of endothelial cells.

[0026] Method: Inoculate the cells in a 6-well plate, and when the cells grow to about 90%, use a pipette tip to make a cross scratch in each well. Wash the scratched cells with PBS, add the medium containing different concentrations of drugs and VEGF, at 37 ° C, 5% CO 2 Cultivate in the incubator until the VEGF group is overgrown

[0027] Results and evaluation: The experimental results are as follows: Figure 2a , Figure 2b As shown, the photographed statistics were observed under a ...

Embodiment 3

[0028] Example 3: Osthole Inhibits Human Umbilical Vein Endothelial Cell Migration Experiment in Boyden Chamber in Vitro

[0029] Purpose and principle: The in vitro Boyden chamber migration experiment of human umbilical vein endothelial cells adopts the Boyden culture chamber of Millipore Company. Under the induction of growth factors, the cells can pass through the 8-micron membrane of the chamber. This experiment can detect the effect of drugs on the endothelial cell membrane penetration ability. influences.

[0030] Method: Put the chamber into a 24-well cell culture plate, add gelatin and incubate for 30 minutes, discard the gelatin after 30 minutes, and wash three times with PBS. Add endothelial cell medium containing VEGF outside the small chamber, insert a certain number of cells into the small chamber, and then add different concentrations of osthole drugs, at 37 ° C, 5% CO 2 Incubate for four hours in the incubator.

[0031] Results and evaluation: After four hours...

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PUM

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Abstract

The invention provides an application of osthole in preparing anti-angiogenic drugs. The osthole can inhibit tumor angiogenesis, arthritis pathological tissue vessel angiogenesis, neovascular eye disease, hemangioma pathological tissue angiogenesis, psoriasis pathological tissue vessel angiogenesis, solid tumor pathological tissue angiogenesis, hemangioma, Kaposi's sarcoma pathological tissue angiogenesis, leukocythemia, lymphadenoma, myeloma blood cancer and Paget's disease angiogenesis. The invention also provides the application of a compound containing effective dose of osthole and pharmacy acceptable ingredients in preparing the anti-angiogenic drugs.

Description

technical field [0001] The invention relates to a new application of a Chinese herbal medicine monomer osthole, in particular to the application of osthole in the preparation of drugs for inhibiting angiogenesis. Background technique [0002] Angiogenesis is the process of generating new blood vessels on the basis of the original vascular structure, and is the result of the interaction between cells and cells, cells and matrix, and cells and cytokines. Angiogenesis occurs during normal physiological processes such as embryonic development and the female menstrual cycle. At the same time, angiogenesis also exists in many pathological processes. [0003] Angiogenesis is a complex process, including five basic processes: ① vascular cells secrete proteolytic enzymes to degrade the vascular basement membrane; ② endothelial cells migrate through the basement membrane to the perivascular matrix; ③ endothelial cells proliferate, adhere to each other and connect; ④ Formation of a l...

Claims

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Application Information

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IPC IPC(8): A61K31/37A61P35/00A61P35/02A61P19/02A61P29/00A61P27/02A61P17/06
Inventor 易正芳代付军张晓丽张京卢彬彬赖力宋雅娟刘明耀
Owner EAST CHINA NORMAL UNIV
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