In-vitro pollen germination liquid medium of balloon flower and method using same to determine pollen viability thereof
A technology of liquid culture medium and in vitro germination, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, measuring devices, etc., to achieve stable and reliable results and easy observation and measurement
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[0027] The invention provides a method for in vitro germination culture of bellflower pollen to measure pollen viability. On the basis of determining the in vitro germination culture system of bellflower pollen, the bellflower pollen is cultured in vitro, and the germination rate of pollen and the number of pollen tubes are measured. Growth conditions, reliable and effective determination of bellflower pollen vigor. The present invention will be described in further detail below in conjunction with the accompanying drawings, which are explanations rather than limitations of the present invention.
[0028] 1. Collection of bellflower pollen
[0029]Platycodon pollen was collected from the 2-year-old platycodon group in the medicinal botanical garden of Shangluo Xiangju Pharmaceutical Company in Shaanxi in mid-July 2008. The collection method is: select robust plants with normal development and no pest damage, and collect them at the flowering stage from 7:00 to 8 am in the morn...
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