Method for preparing seleno-polysaccharide by organic method

Abstract

The invention relates to a method for preparing seleno-polysaccharide by an organic method, which comprises the following steps: (1) placing potentilla anserine polysaccharide and artemisia seed polysaccharide into a reactor, adding N,N-dimethyl formamide into the reactor, and stirring to obtain suspension A; (2) dripping thionyl chloride into selenious acid for reacting, carrying out reduced pressure distillation on the reacted solution to obtain solid matter B; (3) dissolving the solid matter B with the N,N-dimethyl formamide, and adding the mixed solution into the suspension A for reaction to obtain reaction solution C; and (4) dissolving the reaction solution C with distilled water, adjusting the pH value to be 7 with sodium hydroxide, carrying out centrifugation, filling supernatant fluid into a bag filter with the interception quantity of 8,000 to 140,000Da, introducing tap water for dialysis, and carrying out the dialysis with deioned water; and carrying out reduced pressure distillation on the solution in the bag filter, adding ethanol into concentrated solution, standing the mixed solution at room temperature, carrying out centrifugation, collecting deposition on the lower layer and freezing and drying to finally obtain the potentilla anserine polysaccharide or the artemisia seed polysaccharide. The method has the advantages of short reaction time, few byproducts, simple reaction process, high selenium content of products and the like.

Description

technical field [0001] The invention relates to a method for preparing selenized polysaccharides, in particular to an organic method for preparing selenized polysaccharides. Background technique [0002] Selenium is an essential trace element for humans and animals. It is the active component of glutathione peroxidase, which can prevent the oxidative damage of pancreatic islet β cells, make them function normally, promote glucose metabolism, reduce blood sugar and urine sugar, and improve the symptoms of diabetic patients; selenium can protect the retina, enhance The smoothness of the vitreous body improves vision and prevents cataracts; it is also an important element to maintain normal heart function, and has the effect of protecting and repairing the heart body. Current research shows that the reduction of human blood selenium level will lead to the decline of the function of scavenging free radicals in the body, resulting in increased deposition of harmful substances, i...

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Problems solved by technology

Patent CN101104644A discloses a method for preparing polysaccharide selenite and its application. In this method, dextrin, dextran, arabinoxylan and selenium oxyfluoride are added to pyridine, and heated for 0.1-100 hours to obtain A polysaccharide selenite, the highest selenium content can reach 45%, but the reaction time of this method is too long, and the selenium oxyfluoride used is not easy to synthesize, so it is not suitable for large-scale industrial production; patent ZL88103347 authorized a selenized carrageenan The preparation method, the selenized carrageenan prepared by this method has a low selenium content (2500-15000ppm), and generates toxic by-products such as hydrogen selenide; patent CN 101024664A discloses selenium galactose and its chemical synthesis, and this method is Reaction of galactose with organic selenide reagent, the preparation process of this method is complicated, and monosaccharide does not have the pharmacological activity that natural polysaccharide has; Patent CN 101190951A discloses a kind of preparation method of polysaccharide selenate, this method is in dioxygen The selenization reaction of polysaccharides in hexacyclic or toluene adopts heterogeneous reaction. The polysaccharides used are anhydrous dextran or anhydrous astragalus polysaccharides, and selenate is synthesized. The reaction time is about 5 hours, and the selenium content is 769-2250μg / g; patent ZL200410071806.6 authorizes a selenate polysaccharide and its preparation method, the method is to first synthesize selenous acid, selenous acid is reduced to selenic acid, then selenate is introduced into the polysaccharide, and the selenium content is 3mg-200mg / g; Patent CN 1560088A discloses a preparation method of selenized glucomannan, in a Se 6+ Add ethanol and hydrochloric acid to the solution to make a selenized reaction liquid, then add glucomannan to make selenized glucomannan, the selenium content is 125.4-197.3 mg / kg; patent CN1284509A discloses chitosan selenous acid Salt and its preparation method, the method is to react the amino group of chitosan with the added selenous acid, the selenium content of the product is 0-26%

[0005] The selenization technology mentioned in the above patents has the disadvantages of long reaction time, many reaction by-products, generally low selenium content, and complicated reaction process.

Documents at home and abroad do not relate to the synthesis of selenium reagents with selenous acid and thionyl chloride, the method for preparing selenium polysaccharides

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