ELISA testing kit for detecting norketamine and preparation method thereof

Abstract

The invention provides an ELISA testing kit for detecting norketamine (NKET) and a preparation method thereof. The testing kit comprises cleaning solution, color development solution A, color development solution B, stop solution, a coated microplate, a norketamine (NKET) standard, an anti-NKET monoclonal antibody and enzyme labeling goat anti-mouse antibody cryodesiccate, wherein the coated microplate coats a solid phase antigen, the norketamine (NKET) standard is obtained by diluting pure norketamine, and the anti-NKET monoclonal antibody is prepared through preparing a complete antigen of norketamine firstly by utilizing mice immunity. The ELISA testing kit for detecting norketamine uses an enzyme linked immunosorption detection method to calculate NKET content of a sample from a standard curve by detecting absorbance values of norketamine. The invention has simple and convenient operation, rapid, sensitive and accurate detection as well as convenient use and low price of special testing kits, and is suitable for detecting large batch samples.

Description

(1) Technical field [0001] The invention relates to the detection of contraband drugs, belongs to the field of biotechnology, and specifically relates to a test box for enzyme-linked immunoassay of norketamine (NKET) and a preparation method thereof. (2) Background technology [0002] Ketamine, commonly known as "K powder" in my country, has a chemical name of 2-o-chlorophenyl-2-methylamino-cyclohexanone. Attempt to replace phencyclidine (PCP) with it, eliminate the mental side effects of PCP as an anesthetic, and become a new type of anesthetic. Ketamine is a derivative of PCP, which was first synthesized in 1962 and belongs to the category of non-narcotic analgesics. The drug can provide deep analgesia at a sub-anesthesia dose, and it does not have the side effects of inhibiting heart and respiratory function associated with most other conventional anesthetics. It has been clinically tested for many years. [0003] Since the first reported cases of ketamine abuse in San ...

AI Technical Summary

Problems solved by technology

Physical and chemical analysis methods have good sensitivity and specificity, but the operation is cumbersome, requires expensive instruments and equipment, and takes a long time

Although the test strips used in the colloidal gold immunochromatography method overcome the weaknesses of slow detection speed and inability to perform high-throughput detection in traditional instrument detection methods, and do not require professional operation, the detection limit is relatively high.

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