Method for producing genistein protein powder by using natto bacilli to ferment soybean meal

Abstract

The invention relates to a method for producing genistein protein powder by using natto bacilli to ferment soybean meal. The method comprises the following steps: (1) acclimating a fermentation strain: obtaining a natto strain which adapts to soybean meal fermentation culture media of 2 percent, 4 percent, 6 percent, 8 percent and 10 percent (w / v) and generates high beta-glucosidase activity; (2) preparing fermentation seeds: inoculating and culturing natto bacilli which have high yield of beta-glucosidase in soybean meal substrate culture media of various concentrations on inclined planes of test tubes; (3) preparing a fermentation culture medium; forming the fermentation culture medium with a pH value of 7.2-7.4 by 1-3 percent (w / v) glucose, 6-12 percent (w / v) defatted soybean meal, 0.05-0.2 percent (w / v) Ca<+>, 0.02-0.1 percent (w / v) Mg<+>; (4) fermenting: inoculating a seed culture medium with an inoculation amount of 4-10 percent (w / v) in the fermentation culture medium, wherein the liquid-containing rate of a fermentation pot is 40-60 percent (w / v), the fermentation temperature is 32-38 DEG C, the pH value is 7.0-7.5, and the fermentation time is 48-72 h; and (5) spraying and drying. The invention has simple operation and remarkable effect.

Description

technical field [0001] The invention relates to microbial fermentation technology, in particular to a method for producing genistein protein powder by fermenting soybean meal with Bacillus natto. Background technique [0002] Soy genistein is one of the main active substances of soybean isoflavones, a natural plant hormone with unique physiological properties. Many research reports and research experiments have shown that genistein is the most active and functional of isoflavones, and is a non-estrogenic compound with weak estrogenic effects. Its physiological effects are: it has pharmacological effects such as increasing bone density, increasing the length of the tibia, forming bone cells, and preventing bone loss. It is a new type of effective drug for the treatment of osteoporosis; Breast cancer and tumors and other diseases can reduce the incidence of cholesterol, heart disease and cancer, and has the functions of anti-oxidation and inhibition of tyrosine protein kinase...

AI Technical Summary

Problems solved by technology

[0005] One is to use the solvent extraction method; 95% of the soybean isoflavones obtained by this method are still in the bound form, and the content of free aglycone is very small, that is to say, the yield of genistein is extremely low, and it is difficult to regulate various aspects of the human body. The role of physiological function

[0006] The second is to use acid hydrolysis; acid hydrolysis uses strong acid, which is unfavorable to industrialization, and there are many by-products, which pose safety hazards to human life safety and the environment

[0009] At present, the process of producing genistein by using microorganisms is complicated. It needs to undergo microbial fermentation to produce β-glucosidase, and after rough extraction and purification, it acts on the substrate containing soybean isoflavones to convert glycosides into aglycones. substance

The operation process of this method is divided into two steps. The raw material for fermentation production of β-glucosidase is basically different from the substrate of β-glucosidase, and the enzyme extraction process is complicated, the whole operation is complicated, and the production cost is high; Most of the strains are not probiotics, and there are certain restrictions on the application of post-fermentation products