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Endophyte with insecticidal active substances and application in biological control

An endophyte and ergot technology, which is applied in the field of endophytes to achieve the effects of safe and reliable prevention and control, no environmental pollution, and no harm to human health.

Inactive Publication Date: 2010-01-06
THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there is no report on the systematic research on the endophytic bacteria of Drunken Horse Grass. Due to the advantages of easy cultivation, easy control, low production cost, and fast growth, the microorganisms have been developed through methods such as mutagenesis and breeding to achieve large-scale industrialization. Grass endophytes become possible, and their application prospects are very broad

Method used

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  • Endophyte with insecticidal active substances and application in biological control
  • Endophyte with insecticidal active substances and application in biological control
  • Endophyte with insecticidal active substances and application in biological control

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: Isolation, screening and identification of endophyte Claviceps purpurea CGMCC.No3073

[0029] Through the sampling of Drunken Horse Grass in Nanshan Mountain in Urumqi, the samples were divided into root, leaf and seed parts. Sampling adopts 5-point sampling method (east, south, west, north, middle) at every distance of 10 meters, and each point selects 1 strain of test grass, and each strain of grass gets root, leaf, seed (maturity stage), each 2 organ samples were taken. Put the sampled organs of this subspecies into food storage bags and bring them back to the laboratory for isolation.

[0030] Rinse the sample with sterile water first, then soak it in 75% alcohol for 30s, rinse it with sterile water, then soak it with 0.1% mercuric chloride for 1min, rinse it three times with sterile water, add sterile water to grind, absorb the grinding solution Spread on beef extract peptone medium, Gao's No. 1 medium, and PDA medium respectively. Beef extract peptone...

Embodiment 2

[0040] Example 2: Isolation, screening and identification of endophyte Pseudomona fluorescens CGMCC.No3026

[0041] Through the sampling, screening, and separation of Urumqi Nanshan Drunken Horse Grass, the specific method can be as described in Example 1. See attached image 3 , 5

[0042] The obtained GA colony was round with white hyphae. Microscopic observation shows long rod-shaped, medium-growing spores. According to the ninth edition "Bergey's Manual of Systematic Bacterio-logy Identification" ("Bergey's Manual of Systematic Bacterio-logy") and "Common Bacterial System Identification Manual" the shape, size, physiological and biochemical reactions of bacterial strain GA are detected, the present invention The biological characteristics of the GA strains are shown in Table 2.

[0043] Table 2: Physiological and biochemical characteristics of insecticidally active strain GA

[0044]

[0045]

[0046] GA utilizes bacterial universal primers 27F and 1492R:

[0...

Embodiment 3

[0051] Embodiment three: the growth factor of ergot fungus (Claviceps purpurea) CGMCC.No 3073

[0052] The strain PF-2 was inoculated on PDA medium containing different concentrations of salt (NaCl), antibiotic (ampicillin) and pH, and cultured in a 28°C incubator for 7 days. Then the strain PF-2 was connected to the PDA medium, and placed in 4°C, 10°C, 15°C, 20°C, 25°C, 30°C, 35°C, 40°C, 45°C, 50°C, 55°C, 60°C, respectively. Cultivate in an incubator. The results are shown in Table 3.

[0053] Table 3: Effects of temperature, pH, salt, and antibiotics on the growth of strain PF-2

[0054] temperature(°C)

[0055] It can be concluded from Table 3 that the bacterial strain PF-2 can grow in the environment of temperature 20°C-45°C, pH7-8, NaCl concentration 0.5%-8%, ampicillin concentration 50μg / ml-400μg / ml, especially respectively It is most suitable for growth when the temperature is 35°C-40°C, pH7, NaCl concentration 7%-8%, ampicillin concentration 50μg / ml-100μg / ...

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Abstract

The invention discloses endophyte Claviceps purpurea CGMCC.No3073 and Pseudomonas fluorescens CGMCC.No3026 with insecticidal active substances and an application in biological control. The Claviceps purpurea obtains the optimal insecticidal pH of 7 to 8 by separating, screening and culturing in an achnatherum inebrians root, can maintain higher insecticidal activity in the pH range of 7.2-7.4 and has the insecticidal activity in the temperature range of 20 DEG C-37 DEG C; and the Pseudomonas fluorescens has the optimal insecticidal pH of 7 to 8, can maintain higher insecticidal activity in the pH range of 7.2-7.4 and has the insecticidal activity in the temperature range of 20 DEG C-37 DEG C. A bacterial strain of the invention can be widely applied to the biological control field of controlling the damage of cotton bollworms, red spiders, locusts, and the like.

Description

field of invention [0001] The invention relates to the field of biological control, in particular, the invention relates to an endophytic bacterium with insecticidal effect on insect pests such as cotton bollworm, spider mite and locust and application thereof. Background technique [0002] More than 100 years ago, people have discovered that there are microorganisms in the interior of healthy plant tissues, and these microorganisms were later called plant endophytes (Endophytes) in the literature. At present, the more commonly used broad concept of endophytes was proposed by Stone JK, etc., which refers to those fungi or bacteria that live in various tissues and organs of healthy plants during a certain stage or all stages of their life history. The host plant does not (at least temporarily) show outward symptoms, and its endogenous growth can be indicated by histological methods or by isolation of microbial DNA from severely surface-sterilized plant tissue or by direct amp...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N1/20A01N63/02A01N63/04A01P7/04C12R1/39C12R1/645
Inventor 张雪冰张伟高雁史应武娄恺
Owner THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI
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