A kind of insecticidal active substance and its preparation method and application
An active substance and active technology, applied in the field of biological control of plant pests, can solve the problems of high toxicity of chemical pesticides, damage to ecological balance, health impact, etc., and achieve the effect of not endangering human health, not polluting the environment, and preventing and controlling safe and reliable
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Embodiment 1
[0044] Embodiment 1——Containing the preparation of the culture fluid of insecticidal activity metabolite
[0045] (1) First, according to the routine culture method of the microbiology laboratory, pick a small amount of Trichoderma longibrachiae bacteria blocks stored in 20% glycerol tubes and inoculate them on the surface of the PDA plate, and cultivate them at 26°C for four days, as the bacterial classification for the shake flask fermentation culture ,stand-by;
[0046] (2) Prepare 20L of liquid culture medium and distribute it in 100 1000mL Erlenmeyer flasks, each bottle has a filling volume of 200ml, with natural pH, sterilized at 121°C for 20min, and cooled for later use.
[0047] Wherein, the liquid medium is 100g peptone, 600g sucrose, 40g beef extract, 5g magnesium sulfate, 10g potassium dihydrogen phosphate, add distilled water to 20L;
[0048] (3) Under aseptic conditions, scrape the Trichoderma spores on the surface of the PDA plate to be used, inoculate them in a...
Embodiment 2
[0049] Embodiment 2——Preparation of the Crude Extract of Insecticidal Active Substance
[0050] First, the fermentation product of the above-mentioned Trichoderma longibrachiae liquid culture medium is centrifugally filtered to obtain 16L filtrate and 3L mycelium; Alcohol aqueous solution, eluted at a flow rate of 1.6L / h to remove impurities, and then eluted with 2L of 50% alcohol aqueous solution at a flow rate of 1.6L / h to collect insecticidal active ingredients; the obtained mycelium was soaked in 3L of 70% alcohol for 24hrs, filtered, Soak the filter residue in 3L of 70% alcohol for 24hrs, combine the filtrates twice, concentrate the filtrate to remove the alcohol and extract it twice with an equal volume of n-butanol; finally, elute the n-butanol phase active liquid with the previous macroporous adsorption resin with 70% ethanol The liquids were combined and concentrated into 30 g of crude extract.
Embodiment 3
[0051] Embodiment 3 - the preparation of a kind of emulsifiable concentrate
[0052] Get the crude extract 8g that obtains in the embodiment 2, dissolve with 30ml ethanol, add 40ml sec-butyl acetate and 20ml xylene to aid dissolution, finally add 10ml emulsifier SP-4099, obtain 100ml content and be 8% emulsifiable concentrate.
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