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Culture medium for aquatic plant tissue culture and rapid propagation method thereof

An aquatic plant and tissue culture technology, applied in the fields of botany equipment and methods, plant regeneration, horticultural methods, etc., can solve the problems of difficult tissue culture of aquatic plants, and achieve the effect of promoting cell division and expansion, and improving yield and quality.

Inactive Publication Date: 2010-01-06
昆明市水产科学研究所
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

[0004] What the present invention is to solve is the difficult problem of aquatic plant tissue culture, and a kind of tissue culture medium suitable for aquatic plants and its tissue culture rapid propagation method are provided

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0021] Example 1: Select small pearls for tissue culture, first configure the culture medium for subsequent use:

[0022] The culture medium is composed of inorganic nutrients, organic additives, growth regulators, carbon sources, and vitamins, and the contents of each substance are:

[0023] (1) Inorganic nutrients include potassium nitrate 10450Mg / L, ammonium nitrate 9075Mg / L, magnesium sulfate 2035Mg / L, potassium dihydrogen phosphate 935Mg / L, calcium chloride 2420Mg / L, manganese sulfate 1226.5Mg / L, zinc sulfate 473Mg / L, boric acid 341Mg / L, potassium iodide 45.65Mg / L, copper sulfate 1.38Mg / L, cobalt chloride 1.38Mg / L, iron sulfate 5570Mg / L and EDTA7450Mg / L;

[0024] (2) Organic additives include agar 4.5g / L, niacin 4.6Mg / 100ML, glycine 18.4Mg / 100ML;

[0025] (3) Growth regulator substances are 6-BA 0.1Mg / L and NAA 0.1Mg / L;

[0026] (4) The carbon source is glucose 30g / L;

[0027] (5) The vitamins are thiamine 3.68Mg / 100ML, pyridoxine 4.6Mg / 100ML and inositol 920Mg / 100ML....

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Abstract

The invention provides a culture medium for aquatic plant tissue culture and a rapid propagation method thereof and relates to a tissue culture and rapid propagation method in plant biotechnology, in particular to the culture medium for the aquatic plant tissue culture and the rapid propagation method thereof. The culture medium for the aquatic plant tissue culture comprises inorganic nutrient, organic additive, growth regulating substances, a carbon source and vitamins. Each substance in the formula of the culture medium for aquatic plant tissue culture is an essential element for the growth of an aquatic plant, wherein 6-BA is used for promoting the generation of buds and can improve the output and the quality of the aquatic plant; NAA can promote cell division and growth; and 6-BA and NAA simultaneously simulate the growth of single plants and lateral buds. In the method of tissue culture, the procedure of sterilizing external materials of the aquatic plant can inhibit the growth of entogenous bacteria of the aquatic plant in a great degree, thereby the output and the quality of seedlings of the aquatic plant are effectively improved.

Description

technical field [0001] The invention relates to a tissue culture rapid propagation method in plant biotechnology, in particular to a tissue culture medium for aquatic plants and a tissue culture rapid propagation method thereof. Background technique [0002] Tissue culture is a technology that promotes plant cell division and growth through the action of nutrients and hormones in a sterile environment to obtain a large number of clones with the same attributes. Tissue culture has been widely used in seedling cultivation because of its advantages of mass production of seedlings in factory and practical guarantee of selection of fine varieties. [0003] At present, the medium formulations used for terrestrial flower seedlings mainly include MS medium, N6 medium, EDTA chelated particle iron, 6-BA splitting hormone and NAA growth hormone. These medium formulations are quite mature, but they can only be used For the cultivation of terrestrial flower seedlings, it is extremely un...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 彭军杨飏
Owner 昆明市水产科学研究所
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