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Method for preparing D-tagatose and L-tagatose from dulcitol

A technology for tagatose and dulcitol is applied in the field of preparing D-tagatose and L-tagatose from dulcitol, which can solve the problems of high production cost, potential safety hazards and the like, and achieve the effect of high conversion efficiency

Inactive Publication Date: 2009-12-16
SHANGHAI JIAO TONG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In short, relying on the current technology to prepare D-tagatose or L-tagatose has the disadvantages of high production cost and potential safety hazards. Therefore, it is very necessary to develop a cheap, green and efficient D or L-tagatose preparation method

Method used

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  • Method for preparing D-tagatose and L-tagatose from dulcitol
  • Method for preparing D-tagatose and L-tagatose from dulcitol
  • Method for preparing D-tagatose and L-tagatose from dulcitol

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Experimental program
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Effect test

Embodiment 1

[0039] Screening of strains

[0040] 本发明所使用的菌株包括:Acetobacter aceti,Acetobactersuboxydans,Ace tobac ter hansenii,Acetobacter liquefaciens,Acetobacterlovaniensis,Acetobacter pasteurianus,Acetobacter orleanenis,Acetobacter wieringae,Gluconobacter oxydans,Gluconobacter suboxydans,Gluconobacter frateurii,Gluconacetobacter hansenii,Gluconacetobacterliquefaciens,Gluconacetobacter xylinus。 First, the above bacterial strains were cultured in a seed medium at 30° C. and 250 rpm for 24 hours to obtain a seed bacterial liquid. Each liter of seed medium contains: D-dulcitol: 5 grams; glycerin: 5 grams; glucose: 10 grams; yeast powder: 10 grams; dipotassium hydrogen phosphate: 2 grams; One liter, pH 6.0. After the cultivation, 10 ml was added to the fermentation medium, and cultured at 30° C. and 250 rpm for 72 hours. Per liter of fermentation medium contains: D-dulcitol: 30 grams; glycerol: 5 grams; glucose: 20 grams; yeast powder: 15 grams; dipotassium hydrogen phosphate: 2 grams; magne...

Embodiment 2

[0044] Preparation of Immobilized Cells by Alginate Embedding Method and Used to Convert D-Dulcitol to D-Tagatose and L-Tagatose

[0045] Acetobacter suboxydans was cultured in the seed culture medium at 30° C. and 250 rpm for 48 hours to obtain the seed bacterial liquid. Each liter of seed medium contains: D-dulcitol: 5 grams; glycerin: 5 grams; glucose: 10 grams; yeast powder: 10 grams; dipotassium hydrogen phosphate: 2 grams; One liter, pH 6.0. After the cultivation, the supernatant was centrifuged to obtain the bacteria, the bacteria were resuspended with 0.1M sterile sodium alginate solution, sucked into a sterile syringe, and slowly dripped into 0.1M CaCl 2 The solid pellets were precipitated in the solution, placed in a refrigerator at 4°C for 2-4 hours or overnight, then the supernatant was discarded and washed once with sterile water, placed in a proliferation medium at 28-32°C, 300rpm and cultivated overnight. The components of the proliferation medium are: 0.5-5% ...

Embodiment 3

[0047] Preparation of immobilized cells by agarose embedding method and used to convert D-dulcitol to D-tagatose and L-tagatose

[0048] Acetobacter suboxydans was cultured in the seed culture medium at 30° C. and 250 rpm for 48 hours to obtain the seed bacterial liquid. Each liter of seed medium contains: D-dulcitol: 5 grams; glycerin: 5 grams; glucose: 10 grams; yeast powder: 10 grams; dipotassium hydrogen phosphate: 2 grams; One liter, pH 6.0. After the cultivation, centrifuge the supernatant to obtain the bacteria, suspend it with sterile water, add it to the agarose solution (20g / L, w / v) at 40°C, mix well and make it solidify, cut it into small pieces, and add the proliferation medium Incubate overnight at 28-32°C, 300 rpm. The components of the proliferation medium are: 0.5-5% sorbitol, 0.5-5% glycerin, 1-5% glucose and 0.5-5% yeast powder. All the other steps are the same as in Example 2. After the cultivation, HPLC analyzed the conversion rate and the content of ta...

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Abstract

The invention relates to a method for preparing D-tagatose and L-tagatose from dulcitol in the technical field of biology, which comprises the following steps: preparing a culture medium; inoculating acid-producing bacteria into the culture medium to prepare primary seed liquid and secondary seed liquid; culturing the second seed liquid to obtain a water solution containing the D-tagatose and the L-tagatose. The invention also relates to a preparation method adopting resting cell technology, which comprises the following steps: inoculating the acid-producing bacteria into the culture medium to be cultured; centrifugating the cultured bacteria after finishing the to form somatic cells, suspending the somatic cells by using the water solution containing the D-tagatose, and culturing the water solution to obtain the water solution containing the D-tagatose and the L-tagatose. The invention also relates to a preparation method adopting immobilized cell technology, which comprises the following steps: inoculating the acid-producing bacteria into the culture medium to be cultured; centrifugating the cultured bacteria after finishing the culture to obtain somatic cells; obtaining the water solution containing the D-tagatose and the L-tagatose by adopting the immobilized cell technology. The methods of the invention are green and safe, have high conversion efficiency, and can prepare the D-tagatose and the L-tagatose of pharmaceutical grade.

Description

technical field [0001] The present invention relates to a sugar preparation method in the field of biotechnology, specifically a method for preparing D-tagatose and L-tagatose by using dulcitol, and a method for converting D-dulcitol by using resting cells A method for preparing D-tagatose and L-tagatose, and a method for preparing D-tagatose and L-tagatose by using immobilized cells to convert D-dulcitol. Background technique [0002] The application of sucrose in food is still in a dominant position. People eat foods containing sucrose almost every day. It not only serves as a sweetener but also provides a lot of calories. Excessive consumption of sucrose can cause various health problems, especially obesity and Dental caries is especially serious in developed countries. Obesity is closely related to the occurrence of type 2 diabetes. In the United States, almost 9 out of 10 obese patients are diagnosed with different degrees of type 2 diabetes (Diabetes, Obesity and Meta...

Claims

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Application Information

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IPC IPC(8): C12P19/02C12R1/02C12R1/01
Inventor 邓子新程海荣
Owner SHANGHAI JIAO TONG UNIV
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