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Bacillus subtilis microbial agent for preventing and curing alternaria alternate and preparation method thereof

A technology of Bacillus subtilis and tobacco red spot disease, applied in botany equipment and methods, methods based on microorganisms, biochemical equipment and methods, etc., can solve problems such as inappropriate control, rising drug costs, and lack of resistant varieties , to achieve the effect of reducing environmental pollution and pesticide hazards, overcoming pathogen resistance, and ensuring sustainable development

Active Publication Date: 2009-12-09
YUNNAN ACAD OF TOBACCO AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Tobacco red spot disease is a typical airborne disease. At present, agricultural measures such as health care cultivation and chemical pesticides are the main control measures in China, and there is a lack of resistant varieties for regional cultivation; The cost of medication is gradually rising, the control effect is gradually weakening, and pathogenic fungi develop drug resistance, making the prevention and control more difficult
Therefore, it is not suitable to use residual chemical agents for the control of mature diseases such as tobacco red spot disease, and it is necessary to explore new ways such as biological control

Method used

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  • Bacillus subtilis microbial agent for preventing and curing alternaria alternate and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] ——Preparation method of liquid fermentation bacterial agent

[0036] (1) Strain preparation

[0037] Move Bacillus subtilis (Bacillus subtilis) B75 to the test tube slant medium (3g of beef extract, 5g of soybean peptone, 5g of glucose, 17-20g of agar, 1000ml of distilled water, pH 6.8-7.2), and incubate at 30°C for 1 ~ 2 days to obtain test tube seeds.

[0038] (2) Liquid fermentation

[0039] Add 10ml of sterile water to the slant of each test tube, scrape off the bacterial lawn with an inoculation loop, and shake up to form a bacterial suspension, insert sterilized and cooled to 30°C with 0.5% volume ratio, and 200ml culture solution (soybean Peptone 10g, sucrose 5g, yeast extract 2g, mannose 2g, potassium nitrate 1g, ammonium sulfate 1g, tap water 1000ml, pH 6.4-6.8) in a 500ml Erlenmeyer flask, at 30°C at a constant temperature of 180-220 rpm for 36 Hour. It is required to measure the bacterial content of the fermented bacterial liquid by plate counting, and th...

Embodiment 2

[0043] ——Preparation method of solid bacterial agent

[0044] (1) strain preparation: with embodiment 1;

[0045] (2) liquid fermentation: with embodiment 1;

[0046] (3) Formulation

[0047] Add 0.5% sodium carboxymethyl cellulose, 0.2% sodium dodecyl sulfonate, 5% to 10% sclerotium, the mass ratio of diatomite and light calcium carbonate is 9:1, mix well, beat, Spray drying to make wettable powder. 100g / bag into plastic or aluminum plastic bags. Its packaging should undergo strict inspection and meet the packaging standards for wettable powder pesticides.

[0048] Measurement of finished product quality indicators: the determination of bacteria content, water content, suspension rate, and fineness is carried out according to the national enterprise standard (Q / KWL02-2003).

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Abstract

The invention discloses a bacillus subtilis microbial agent for preventing and curing alternaria alternate. The production bacterial strain of the microbial agent is classified and named as the Bacillus subtilis B75 with the preservation number CGMCC No.1700. The bacterial strain is moved to the inclined culture medium of a test tube and cultured with a constant temperature 30 DEG.C+ / -1 DEG C for 1-2 days to obtain a test tube strainThe test tube strain is cultured by means of liquid shake-flask fermentation for 30-36 hours to obtain liquid fermenting microbial agent or solid microbial agent. The experiment indicates that the microbial agent as a good application prospect for preventing and curing the alternaria alternate.

Description

technical field [0001] The invention belongs to the technical field of biological pesticides, and in particular relates to a Bacillus subtilis bacteria agent for preventing and treating tobacco red spot disease. At the same time, the invention also relates to a preparation method of the bacterial agent. Background technique [0002] Tobacco Brown Spot Disease (Tobacco Brown Spot Disease) is an important leaf disease in the late mature stage of tobacco leaves. It generally occurs in various smoking areas and is the main disease of tobacco in my country. Statistics on the occurrence of tobacco diseases and insect pests in the country from 2006 to 2007 show that there are 7 provinces, including Yunnan, Guizhou, Sichuan, Hubei, Hunan, Guangdong, and Shaanxi, with an annual incidence area of ​​more than 50,000 mu of red spot disease, and the annual incidence area of ​​the country is 1.3 to 1.8 million. Mu, the output loss was more than 10,000 tons, and the output value loss was ...

Claims

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Application Information

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IPC IPC(8): A01N63/02A01P3/00C12N1/20C12R1/125
Inventor 方敦煌宋春满逄涛雷丽萍夏振远师君丽白江兰
Owner YUNNAN ACAD OF TOBACCO AGRI SCI
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