Immune colloidal gold test card for testing high-risk type human papillomavirus and test method thereof

Abstract

The invention provides an immune colloidal gold test card for testing high-risk type human papillomavirus. A test strip thereof consists of a back lining, a sample pad, a combining pad, an NC membrane and a water-absorbing pad; the upper middle part of the back lining is provided with the NC membrane; the two ends of the NC membrane are respectively connected with the combining pad and water-absorbing pad in a neighboring way; the sample pad is arranged above the combining pad; a gold-labelled antibody G-Ab1 is sprayed on the combining pad; the coated gold-labelled antibody G-Ab1 is the monoclonal antibody of the high-risk type human papillomaviru virus HPV16; and the NC membrane is respectively sprayed with the polyclonal antibody Ab2 of the high-risk type human papillomaviru virus HPV16 and the antibody of the gold-labelled antibody, namely the IgG antibody of a rabbit antimouse. The invention applies the technology of immune colloidal gold into the quick testing of the high-risk type human papillomaviru virus; moreover, the test strip for quick testing the high-risk type human papillomaviru virus does not need a test device, thus being convenient for the application to the clinic quick diagnosis and suitable to hospitals of various grades to screen cervical carcinoma.

Description

technical field [0001] The invention relates to immune colloidal gold test card technology, in particular to a human papillomavirus immune colloidal gold test card capable of rapidly diagnosing high-risk human papillomavirus infection type 16 and a testing method thereof. Background technique [0002] Human papilloma virus (HPV16) is an epitheliophilic virus widely distributed in humans and animals with high specificity. HPV infection usually causes genital warts, cervix and genitourinary tract cancer. HPV virus is the culprit of almost all cervical cancers. Men will not die after being infected with the virus, but they will pass it on to their female partners through sex, skin-to-skin contact, etc. The initial symptoms of HPV infection are fine and dense verrucous papillomas on the hands, lower abdomen or genital area. The incubation period of the virus can be as long as 15 years, the lesions develop slowly, and not all infected people will have these symptoms on the body...

AI Technical Summary

Problems solved by technology

In areas where the Pap smear method is used reasonably, the incidence rate of cervical cancer is reduced by 70%, but there are two problems in this method: (one) the false negative rate is high

(2) The current standard for Pap smear classification is the revised Bethesda classification system. Although this system is widely accepted, it has some defects. A large number of Pap smears are reported as undetermined atypical squamous cells every year. , how to properly handle such patients, save resources and increase the screening rate is a thorny issue

D. The false negative rate of PCR detection method is high, the operation of typing is cumbersome, the sample size of one-time detection is limited, and it is not convenient to identify unknown types, thus limiting its application in large-scale screening; E. Development in recent years The advanced DNA chip technology has the advantages of high parallelism, diversity, miniaturization and automation, and has been widely used in DNA research fields such as gene positioning, DNA sequencing, mutation detection, gene screening, and gene diagnosis.

Currently used HPV detection methods have their own advantages and disadvantages, and all have common limitations: time-consuming and complicated procedures

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