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Method for detecting tumour cells by adopting microelectrode array impedance biosensor chip

A microelectrode array, biosensor technology, applied in material impedance, instruments, measuring devices, etc., can solve the problems of difficult to guarantee processing quality, complicated manufacturing process, complicated data acquisition and processing work, etc., and achieve high sensitivity and simple detection method. , reduce the possible effect of missed detection

Inactive Publication Date: 2009-11-11
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The sensor chip in this invention adopts a cantilever beam structure, the manufacturing process is complicated, the processing quality is difficult to guarantee, and the detection information needs to be converted into electronic information to be detected, which requires complicated data collection and processing.

Method used

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  • Method for detecting tumour cells by adopting microelectrode array impedance biosensor chip
  • Method for detecting tumour cells by adopting microelectrode array impedance biosensor chip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1) A microelectrode array impedance biosensor chip composed of four microelectrode array detection units is adopted, and each microelectrode array detection unit includes a five-layer structure of a support layer, a base layer, a micro-sensing electrode layer, an insulating layer, and a liquid reservoir ; The micro-sensing electrode layer has 16 channels of micro-sensing electrodes, the size range of each micro-sensing electrode is 10 μm, and the distance between the micro-sensing electrodes is 10 μm;

[0028] 2) Extract 1 ml of sputum or blood sample, add it to the microelectrode array impedance biosensor chip reservoir, after liquefaction treatment, extract 1ul type A specific protein antigen and add it to the microelectrode array impedance biosensor chip reservoir In the liquid pool, the tumor cells in the sputum or blood samples in the liquid pool are repaired by the type A specific protein antigen to form a type A specific protein antigen-tumor cell biological combi...

Embodiment 2

[0038] 1) A microelectrode array impedance biosensor chip composed of four microelectrode array detection units is adopted, and each microelectrode array detection unit includes a five-layer structure of a support layer, a base layer, a micro-sensing electrode layer, an insulating layer, and a liquid reservoir ; The micro-sensing electrode layer has micro-sensing electrodes with 36 channels, the size range of each micro-sensing electrode is 30 μm, and the distance between micro-sensing electrodes is 20 μm;

[0039] 2) Extract 3ml of sputum or blood sample, add it to the microelectrode array impedance biosensor chip reservoir, after liquefaction treatment, extract 3ul type A specific protein antigen and add it to the microelectrode array impedance biosensor chip reservoir In the liquid pool, the tumor cells in the sputum or blood samples in the liquid pool are repaired by the type A specific protein antigen to form a type A specific protein antigen-tumor cell biological combinat...

Embodiment 3

[0043] 1) A microelectrode array impedance biosensor chip composed of four microelectrode array detection units is adopted, and each microelectrode array detection unit includes a five-layer structure of a support layer, a base layer, a micro-sensing electrode layer, an insulating layer, and a liquid reservoir ; The micro-sensing electrode layer has 100 channels of micro-sensing electrodes, the size range of each micro-sensing electrode is 50 μm, and the distance between the micro-sensing electrodes is 30 μm;

[0044] 2) Extract 5ml of sputum or blood sample, add it to the microelectrode array impedance biosensor chip reservoir, after liquefaction treatment, extract 5ul type A specific protein antigen and add it to the microelectrode array impedance biosensor chip reservoir In the liquid pool, the tumor cells in the sputum or blood samples in the liquid pool are repaired by the type A specific protein antigen to form a type A specific protein antigen-tumor cell biological combi...

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Abstract

The invention relates to a method for detecting tumour cells by adopting a microelectrode array impedance biosensor chip. The method comprises the steps of: adopting nanometer immunity magnetic microballoon concentration separating technology to capture tumour cells from sputamentum or blood samples to form magnetic miroballoon-tumour cell biological magnetic combination; and utilizing magnetic force generated by a magnet to lead the magnetic miroballoon-tumour cell biological magnetic combination to be instantly absorbed on the surface of the microelectrode array impedance biosensor chip, arousing principles of changing impedance characteristic of micro-sensing electrode and adopting an impedance analyzer to conduct parallel scanning testing to the impedance characteristic of the micro-sensing electrode so as to determine whether the tumour cells exist. The invention provides a non-damage, quick, simple and convenient tumour cell detection method, and can be applied to the tumour early diagnosis and treatment, and can be used for diagnosing and monitoring the shifting and recurring of cancer cells of clinical tumour patients and carrying out wide prediction for major diseases, and also can be applied to the fields such as food detection, drug screening and the like.

Description

technical field [0001] The invention relates to a method for detecting tumor cells by using a microelectrode array impedance biosensor chip. It can be used in food testing, drug screening, disease diagnosis and treatment and other fields. It belongs to the interdisciplinary field of biomedical engineering and electronic sensing technology. Background technique [0002] Generally speaking, the methods for the diagnosis of lung cancer mainly include pathology, imaging, immunology, and molecular biology. The samples tested include sputum, mass, pleural effusion and blood. As far as the current imaging detection level of hospitals in our country is concerned, it is generally believed that once a lung cancer mass is found, in most cases the mass is ≥ 1 cm, and the patient's disease course is no longer in the early stage. Among the above-mentioned cytological examinations, the application of blood and sputum cell examinations is the most common. The detection of sputum cancer c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/02G01N33/574G01N33/543
Inventor 惠春邢玉梅
Owner SHANGHAI JIAO TONG UNIV
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