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Saccharomyces cerevisiae, dry yeast rich in reduced glutathione and preparation method thereof

A technology of glutathione and Saccharomyces cerevisiae strains, applied in the direction of microorganism-based methods, biochemical equipment and methods, fungi, etc., can solve the problems of low glutathione yield and product activity reduction, and achieve fixed assets Less investment, less activity loss, and lower production cost

Active Publication Date: 2012-07-11
ANGELYEAST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Low glutathione yield during purification due to many steps
In addition, due to the conversion of part of reduced glutathione to oxidized glutathione, the activity of the product is reduced

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035]In this example, a yeast strain (CCTCC M 205130) with high content of reduced glutathione and stable performance was screened by traditional ultraviolet mutagenesis method.

[0036] The ultraviolet mutagenesis process is as follows: first culture the yeast strain (Ange Z2.2 Angel Company Collection Center number) on a slant medium (cultivate at 30°C for 2 to 3 days), take the slant bacteria, and dilute it with normal saline Yeast cell concentration to 10 6 ~10 7 pcs / ml, take 5ml of bacterial suspension in a sterile empty plate, and carry out magnetic stirring, irradiate for 15-30s at a distance of 20-30cm from a UV lamp (30W, 2537A wavelength), take 100ul of the treated bacterial solution in 50ml of fresh In the liquid seed medium, culture at a constant temperature of 30°C and avoid light for one day. The intermediate culture solution was spread on a screening plate (containing 500ug / ml ethionine) according to the concentration gradient for screening, and a strain (CCT...

Embodiment 2

[0040] In this example, the glutathione-rich dry yeast is fermented as follows: first, the glutathione-rich yeast strain (CCTCC M 205130) is placed on a slant containing wort agar at 30° C. for 40 hours, Inoculate and cultivate 50 hours in a culture bottle with 12 Bahrain's wort as a medium, then inoculate in a Ponzi tank with 12 Bahrain's wort and molasses mixture (1:1 mix) as a medium and inoculate for 50 hours at 30°C, and then inoculate with The molasses with sugar content of 5% (percentage by weight) and pH of 4.8 were inoculated and cultivated in a pure culture tank for 20 hours as a medium, and then the molasses with a sugar content of 5% (percentage by weight) was used as the main raw material, adding 2% of (NH 4 ) 2 SO 4 and 1% ammonium hydrogen phosphate, adjust the pH value to be 4 and inoculate and cultivate in the seed tank for 30 hours, the cultivation temperature is 32 ℃, and the air volume is 1 gram of O 2 / 1 gram of yeast for about 1 minute, then use molas...

Embodiment 3

[0042] In this example, the glutathione-rich dry yeast was fermented as follows: first, the glutathione-rich yeast strain (CCTCC M 205130) was placed on a slant containing wort agar at 30° C. for 45 hours, Inoculate and cultivate in a culture bottle with wort as a medium for 45 hours, then use 12% Bahrain wort and molasses mixture (mixed according to 1:1) as a medium to inoculate in a Ponzi tank at 30°C for 40 hours, and then inoculate with 5 % (percentage by weight) molasses was culture medium inoculated and cultivated for 18 hours in a pure culture tank, and then molasses with a sugar content of 5% (percentage by weight) was the main raw material, adding 2% (NH 4 ) 2 SO 4 and 1% ammonium hydrogen phosphate, adjust the pH value to be 4 and inoculate and cultivate in the seed tank for 26 hours, the cultivation temperature is 30 ℃, and the air volume is 0.5 gram O 2 / 1 gram of yeast for about 1 minute, then use molasses with a sugar content of 5% (percentage by weight) as th...

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PUM

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Abstract

The invention provides a saccharomyces cerevisiae rich in reduced glutathione, having a preserving number of CCTCC M 205130, and also provides dry yeast rich in reduced glutathione and a preparation method thereof, wherein the dry yeast rich in reduced glutathione is produced by fermenting the saccharomyces cerevisiae rich in reduced glutathione. The saccharomyces cerevisiae CCTCC M 205130 with higher content of reduced glutathione and stable property is selected through an ultraviolet mutagenesis method. The saccharomyces cerevisiae is directly used together with yeast cells without extracting and purifying glutathione so as to obtain the dry yeast rich in reduced glutathione. The invention has low production cost, simple process and less investment of permanent assets. Glutathione is notextracted and purified so that the problem of loss of glutathione does not exist, and the activity loss of glutathione is little.

Description

technical field [0001] The invention relates to a baker's yeast, in particular to a dry yeast rich in reduced glutathione and a preparation method thereof. Background technique [0002] Glutathione is a tripeptide compound formed by condensation of glutamic acid, cysteine ​​and glycine through peptide bonds. The chemical name is: -L-glutamyl-L-cysteine-glycine. There are two forms of glutathione in nature, one is reduced glutathione and the other is oxidized glutathione. What really works is reduced glutathione, which plays an important role in anti-oxidation, scavenging free radicals, detoxification, enhancing immunity, delaying aging, anti-cancer, anti-radiation, etc., and is an important functional factor. It is widely used in food industry and pharmaceutical industry. [0003] At present, glutathione is mainly obtained through direct extraction and purification of raw materials, chemical synthesis, enzymatic synthesis and fermentation synthesis. The production of pu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/18C12R1/865
Inventor 俞学锋李知洪余明华姚娟余华顺郑国斌
Owner ANGELYEAST CO LTD
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