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Enzymic measuring reagent of alpha-L-fucosidase resistant to heparin interference and using method thereof

A technology of fucosidase enzyme and anti-interference agent, which is applied in the biological field, can solve the problems of high positive rate and achieve the effects of high sensitivity, excellent stability and good stability

Inactive Publication Date: 2009-11-04
SICHUAN MACCURA BIOTECH CO LTD
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Problems solved by technology

So far, the determination of serum alpha-fetoprotein level is still the most specific tumor marker for the clinical diagnosis of primary liver cancer. However, in the cases of primary liver cancer with negative alpha-fetoprotein, about 70-85% have AFU positive results, and a small The positive rate of serum AFU in patients with liver cancer (tumor diameter < 3CM) was higher than that of alpha-fetoprotein

Method used

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  • Enzymic measuring reagent of alpha-L-fucosidase resistant to heparin interference and using method thereof
  • Enzymic measuring reagent of alpha-L-fucosidase resistant to heparin interference and using method thereof
  • Enzymic measuring reagent of alpha-L-fucosidase resistant to heparin interference and using method thereof

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Embodiment 1

[0028] (1) Sample preparation: samples are fasting serum or plasma (heparin anticoagulation, 0.1mg heparin can anticoagulate 1.0ml blood; EDTA anticoagulation, 1.8mgEDTA can anticoagulate 1.0ml blood) or body fluid (centrifugation is required for clarification). The samples should be transported and stored under low temperature conditions. The α-L-fucosidase in the samples can be stable for 8 hours at room temperature and 7 days at 2°C to 8°C. Stable for 2 months when stored at -20°C.

[0029] (2) Prepare the α-L-fucosidase diagnostic reagent according to the following ingredients and dosage:

[0030] R1:

[0031] 2-Morpholineethanesulfonic acid (MES) 0.10-100g / L

[0032] Phosphate: 0.10-100g / L

[0033] Stabilizer: 0.1-10g / L

[0034] R2:

[0035] 2-Morpholineethanesulfonic acid (MES): 0.1-100g / L

[0036] EDTA-Na 2 : 0.01-10g / L

[0037] Stabilizer: 0.1-10g / L

[0038] NaCl: 0.05-25g / L

[0039] MG-CNPF: 0.05-20g / L

[0040] Alcohol: 1-100g / L

[0041] Anti-interference ...

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Abstract

The invention relates to an enzymic measuring reagent of alpha-L-fucosidase resistant to heparin interference and a using method thereof. The reagent of the invention is a liquid dual reagent and has excellent resistance to heparin interference and excellent stability. The reagent can be used on a semi / full automatic biochemical analyzer by adopting a speed colorimetric technique. The product uses a new substrate and the formulation is added with anti-interference agent resistant to heparin interference. The reagent has the advantages of high sensibility and strong resistance to interference. The product is added with selected stabilizer so that the reagent has excellent stability without influence on performance after the product is stored (keep out of light and sealed) for 12 months at the temperature of 2-8 DEG C. The reagent can be widely used for early diagnosis of primary carcinoma of liver, health examination, liver disease general survey and the like.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an anti-heparin interference α-L-fucosidase enzymatic assay reagent and a use method thereof. Background technique [0002] α-L-fucosidase (Fucosidase, AFU, α-L-fucoside-fucose hydrolase, EC3.2.1.51) belongs to lysosomal acid hydrolase, exists in human liver, brain, lung , kidney, pancreas, fibroblasts and leukocytes in lysosomes, serum and urine also contain a certain amount of enzymes. The main physiological function of AFU is to participate in the catabolism of bioactive macromolecules such as fucosyl-containing glycoproteins and glycolipids. In patients with hereditary AFU deficiency, the reaction of hydrolyzing fucosyl from the above-mentioned biological macromolecules is blocked, causing fucose accumulation disease, and most children die at the age of 5 to 6. The determination of serum AFU was mainly used in the diagnosis of fucose storage diseases in the past. Si...

Claims

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Application Information

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IPC IPC(8): G01N33/50G01N35/00G01N21/31G01N33/576
Inventor 王一赵喻王保宁
Owner SICHUAN MACCURA BIOTECH CO LTD
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