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New application of fructus ligustri lucidi in preparing medicines for adjusting human body vitamin D3 metabolism and medicines for promoting calcium absorption and medicinal preparation thereof

A technology of Ligustrum lucidum and vitamins, applied in the direction of medical preparations containing active ingredients, metabolic diseases, drug combinations, etc.

Inactive Publication Date: 2009-11-04
THE HONG KONG POLYTECHNIC UNIV SHENZHEN RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Judging from the existing professional publications and patent literature, there is no effect of Ligustrum lucidum on vitamin D 3 Metabolism and description of its active components that regulate calcium balance in the body

Method used

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  • New application of fructus ligustri lucidi in preparing medicines for adjusting human body vitamin D3 metabolism and medicines for promoting calcium absorption and medicinal preparation thereof
  • New application of fructus ligustri lucidi in preparing medicines for adjusting human body vitamin D3 metabolism and medicines for promoting calcium absorption and medicinal preparation thereof
  • New application of fructus ligustri lucidi in preparing medicines for adjusting human body vitamin D3 metabolism and medicines for promoting calcium absorption and medicinal preparation thereof

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0029] Experimental Example 1: Ligustrum lucidum total extract acts on vitamin D 3 Metabolic animal experiments

[0030] 1.1 Materials and methods

[0031] 1.1.1 Animals and materials

[0032] Animals: 20 11-month-old SPF female SD rats (average body weight 389 g), purchased from Guangdong Medical Experimental Animal Center (animal certificate number: SCXK (Guangdong) 2003-0002).

[0033] Medicinal materials: Ligustrum medicinal materials are collected from Xuyi, Jiangsu

[0034] 1.1.2 Experimental method

[0035] 1.1.2.1 Preparation of the total extract of Ligustrum lucidum: Mix crushed Ligustrum lucidum with 6 times the volume of 70% ethanol, reflux extraction twice, each time for 5 hours, collect the extract, and after coarse filtration, the filtrate is concentrated by rotary evaporation. After freeze-drying, a dry powder was obtained for subsequent use.

[0036] 1.1.2.2 Drug treatment: After the animals have adapted to the environment for two weeks, the animals were r...

experiment example 2

[0048] Experimental Example 2: Cell Experiment

[0049] 2.1 Routine cell culture

[0050] 2.1.1. Materials used in the experiment

[0051] HKC-8 cells

[0052] DMEM / F12 culture medium (Cat.NO.11320) GIBCO company

[0053] Antibiotics (10×, containing penicillin, streptomycin) GIBCO Corporation

[0054] PBS(Cat.NO.20012) GIBCO

[0055] Fetal bovine serum (Cat.NO.16000-044) American Life Technologies company

[0056] Trypsin (Cat.NO.15400-054) American Life Technologies

[0057] Insulin Sigma

[0058] Transferrin Sigma

[0059] Na 2 SeO 3 Sigma Corporation

[0060] T3 Sigma

[0061] Epidermal growth factor Sigma

[0062] Hydrocortisone Sigma

[0063] 2.1.2 Experimental method

[0064] The cells were cultured in DEME / F12 medium (containing 5% FBS+1×antibiotics), and the culture dish was placed in a constant temperature incubator (37°C, 5% CO 2 ). When the confluence of adherent cells reaches 80%-90%, they can be passaged. Absorb ...

experiment example 3

[0077] Experimental example 3 Animal experiments

[0078] 3.1 Materials and methods

[0079] 3.1.1 Animals and materials

[0080] Animals: 32 4-month-old SPF-grade female SD rats (average body weight 389 g), purchased from the Experimental Animal Center of the Chinese University of Hong Kong.

[0081] Medicinal materials: Ligustrum medicinal materials are collected from Xuyi, Jiangsu

[0082] 3.1.2 Experimental method

[0083] 3.1.2.1 Separation of the total extract of Ligustrum lucidum: raw medicinal materials of Ligustrum lucidum, add 70% ethanol to decoct three times, 2 hours each time, combine the decoction, filter, concentrate the filtrate to remove ethanol, add appropriate amount of hot water to disperse, and use The same volume of ethyl acetate was extracted three times, the filtrate was concentrated to remove ethyl acetate, and freeze-dried to obtain the ethyl acetate fraction; the remaining water-soluble parts were concentrated to a thick paste with a relative dens...

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Abstract

The invention discloses new pharmaceutical application of fructus ligustri lucidi and fructus ligustri lucidi extracts in preparing medicines for adjusting human body vitamin D3 metabolism and medicines for promoting calcium absorption, and also discloses granules and capsules for adjusting human body vitamin D3 metabolism and promoting calcium absorption; the medicines for adjusting human body vitamin D3 metabolism adopt ethanol extracts of dry and ripening fructus ligustri lucidi or fat-soluble components in the ethanol extracts; the medicines for promoting calcium absorption adopt water-soluble components in the ethanol extracts of the fructus ligustri lucidi. The application of the single medicines prepared by fructus ligustri lucidi in adjusting human body vitamin D3 metabolism and promoting calcium absorption and corresponding medicinal preparations have the advantages of curative effects, simple preparation process and relatively low cost.

Description

technical field [0001] The invention relates to privet fruit regulating vitamin D in human body 3 Drugs that metabolize and promote calcium absorption, specifically relate to new medical uses of Ligustrum lucidum and Ligustrum lucidum extract, pharmaceutical preparations and preparation methods thereof. Background technique [0002] Osteoporosis is a metabolic bone disease characterized by decreased bone mass, microarchitectural deterioration of bone tissue, and increased bone fragility and fracture risk. There are nearly 90 million osteoporosis patients in my country, and 20 million of them have fractures. With the arrival of an aging society, its incidence rate is increasing year by year, which has brought serious consequences to individuals, families, government and society. 1,25 Hydroxylated Vitamin D 3 [1,25(OH) 2 D. 3 , calcitriol] is the active metabolite of vitamin D, in the pathogenesis of osteoporosis, it will involve the reduction of active metabolites of cal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61K9/16A61K9/48A61P3/00A61P3/14A61K131/00
Inventor 董晓莉黄文秀车镇涛
Owner THE HONG KONG POLYTECHNIC UNIV SHENZHEN RES INST
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