Preparation method of anti-influenza A H1N1 virus specific IgY and related formulation thereof

An influenza virus, avian influenza virus technology, applied in the preparation method of peptide, antiviral agent, antiviral immunoglobulin, etc., can solve the problem of unstoppable H1N1 influenza virus, central nervous system side effects, failure to detect, etc. problems, to achieve the effect of good immune conditioning and inhibition, and elimination of viral infections

Inactive Publication Date: 2009-10-14
深圳雅臣生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] 3. Wear a mask: Because the H1N1 influenza virus is as small as the general influenza virus, the general mask cannot block the H1N1 influenza virus at all.
On April 30, 2009, the flight from the epidemic area Mexico to Shanghai had a H1N1 influenza virus carrier on board. After two hours of body temperature testing at Shanghai Airport; however, such strict multiple body temperature testing; still failed any abnormality detected
However, this type of drug has obvious side effects of the central nervous system, and there have been many cases of neurological symptoms caused by taking "Tamiflu" in Japan
Moreover, it has also been clinically proven that taking such drugs is prone to the spread of drug-resistant poison beads; thus, its clinical application is limited.
In addition, this drug has only a certain therapeutic effect and has no preventive effect at all.

Method used

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  • Preparation method of anti-influenza A H1N1 virus specific IgY and related formulation thereof
  • Preparation method of anti-influenza A H1N1 virus specific IgY and related formulation thereof
  • Preparation method of anti-influenza A H1N1 virus specific IgY and related formulation thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0061] 7. Preparation of Crude Extract

[0062] Firstly, according to the different birds to be immunized and the antigens used for immunization, the immunized eggs are classified and coded. Wash the immune eggs with running water, scrub and disinfect with alcohol, break the immune eggs with an egg beater, filter the egg whites with a yolk sieve, leave the egg yolks, and stir evenly; add distilled water to dilute and mix according to 4-6 times the volume of the egg yolk liquid Evenly, adjust the pH to 5.5-6.0 with 1.0N HCI solution.

[0063] The diluted solution with adjusted pH value is further fully stirred evenly, then cooled to 2-6°C, and left to stand for 12 hours to 24 hours; the diluted solution is centrifuged at 10,000rpm for 20 minutes; the supernatant obtained from separation is ultrafiltered Concentrate by ultrafiltration in a container for 10-20 times; then add sodium alginate solution, stir until cloudy; then add 1.0% CaCl 2 solution, stir evenly, let stand at 4...

experiment example 1

[0083] Experimental example 1 : Detection of specific IgY of anti-mutant influenza A (H1N1) virus and specific IgY of anti-influenza secondary infection bacteria

[0084]Using SDS-PAGE (sodium dodecyl sulfate-polypropylene gel) electrophoresis assay, the specific IgY of different anti-variant influenza A H1N1 influenza virus and the specificity of anti-influenza secondary infection bacteria prepared according to the above process respectively The crude IgY extract was detected, and the result contained 45-52% IgY. The crude IgY extract was passed through the column twice to obtain pure IgY. After SDS-PAGE analysis, the purity reached PAGE pure, as shown in the table below:

[0085] Pure IgY

experiment example 2

[0086] Experimental example 2 : Activity detection of specific IgY against mutated influenza A (H1N1) virus and specific IgY against bacteria secondary to influenza infection

[0087] The anti-variant H1N1 influenza virus-specific IgY and the anti-influenza secondary infection bacteria-specific IgY prepared by the technology invented by the inventors were tested by "ELISA" (enzyme-linked immunosorbent assay).

[0088] The test results show that, among the two different anti-variant H1N1 influenza virus-specific IgYs prepared, the antibody binding titer of type I to the influenza A H1N1 influenza virus has reached above 1:1024. And the antibody binding titer of type II to influenza A H1N1 virus also reaches above 1:512. Similarly, the anti-influenza secondary infection bacterial specific IgY antibody titers to representative pneumococci, group A group B hemolytic streptococcus, Staphylococcus aureus, and Haemophilus influenzae were all above 1:256.

[0089] Detection results...

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Abstract

The invention discloses a preparation method of an anti-influenza A H1N1 virus specific IgY and related formulations thereof. The method comprises the steps of preparing a plurality of types of complex antigens aiming at influenza A H1N1, using the complex antigens for immunizing laying hen, utilizing the immune egg yolk for preparing specific IgY crude extract dry powder, conducting purification, removing all types of bacterial virus by filtration, and then preparing the anti-variant influenza A H1N1 virus specific IgY, anti-influenza secondary infection bacterial specific IgY, or anti-variant influenza A H1N1 virus specific complex IgY. In addition, the invention makes the anti-variant influenza A H1N1 virus specific IgY into nanoliposomes, and the obtained nanoliposome anti-variant influenza A H1N1 virus specific IgY enhances healing efficacy. The anti-influenza A H1N1 virus specific IgY can be utilized for preparing various formulations which then can prevent and teat the influenza A H1N1 of human and animals fundamentally; and the invention is more convenient, more economic, safer and more effective than the control method used at the present time.

Description

technical field [0001] The invention relates to an antibody and a novel preparation thereof, in particular to a method for preparing anti-H1N1 influenza virus-specific IgY and related preparations. Background technique [0002] The influenza that first broke out in Mexico in April 2009 and then spread rapidly to many countries and regions in the world was caused by a type A H1N1 [A(H1N1)] influenza virus. This type A H1N1 influenza virus is a mixture of human influenza virus, North American avian influenza virus, and North American, European and Asian swine influenza virus, including human influenza virus, North American avian influenza virus, and North American, European and Asian swine influenza virus. The genetic component of the Asian swine flu virus is a new type of virus with genetic mutations. Most worryingly, this novel virus has spread from person to person. According to the similar characteristics of blood relatives between pigs and humans, it shows that the immu...

Claims

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Application Information

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IPC IPC(8): C07K16/10C07K16/02C07K1/14C07K16/12A61K39/42A61K39/40A61K9/127A61P31/16
Inventor 包晟杨荣鉴包海威
Owner 深圳雅臣生物科技有限公司
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