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Effective component of artemisia leaves and preparation method thereof

An effective component, Artemisia argyi leaf technology, applied in the field of traditional Chinese medicine extracts for the treatment of tumor diseases, can solve the problems of side reactions, involving normal cells, etc.

Inactive Publication Date: 2009-10-07
TIANJIN TASLY PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the treatment of malignant tumors in the industry is mainly based on surgery, radiotherapy, and chemotherapy, but many chemical anticancer drugs often involve normal cells when they act on target cells, causing serious side effects

Method used

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  • Effective component of artemisia leaves and preparation method thereof
  • Effective component of artemisia leaves and preparation method thereof
  • Effective component of artemisia leaves and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0100] Embodiment 1 Preparation of Artemisia argyi effective components

[0101] Artemisia argyi leaves are pulverized, passed through a 20-mesh sieve, added 8 times the amount of ethyl acetate:ethanol=1:1, heated and refluxed for 1 hour, extracted 2 times, added 8 times the amount of 70% ethanol to the dregs, heated and refluxed for 1 hour, extracted 2 times Once, the filtrate was combined to obtain the extract, after the extract was concentrated, the sample was dissolved with 5% ethanol, and the ODS-C18 column was crossed. At first, 1250ml of 5% ethanol was used as the mobile phase, and then 1250ml of 50% ethanol was replaced as the mobile phase to obtain a wash. Remove the liquid, and continue to separate the eluent by preparative liquid chromatography. Separation conditions: the chromatographic column is an Agilent preparative column (Zorbax SB-C18; 21.2mm×250mm), the mobile phase is water A and acetonitrile B, and the gradient elution procedure is as follows

[0102]

...

Embodiment 2

[0104] Example 2 Analysis of the active components of Folium Artemisiae Argyi

[0105] Chromatographic conditions Chromatographic column Agilent Zorbax SB-C18 column (4.6mm × 150mm, 5 μm); using gradient elution, mobile phase A is 0.2% glacial acetic acid aqueous solution, mobile phase B is acetonitrile solution containing 0.2% glacial acetic acid; gradient elution The procedure is as follows: at 0 minutes, mobile phase A is 90% 0.2% glacial acetic acid aqueous solution, mobile phase B is 10% 0.2% glacial acetic acid in acetonitrile solution; at 10 minutes, mobile phase A is 50% 0.2% glacial acetic acid Aqueous solution, mobile phase B is 50% 0.2% glacial acetic acid acetonitrile solution; 30 minutes, mobile phase A is 5% 0.2% glacial acetic acid aqueous solution, mobile phase B is 95% 0.2% glacial acetic acid acetonitrile solution; 35 In minutes, the mobile phase A was 5% 0.2% glacial acetic acid in water, and the mobile phase B was 95% 0.2% glacial acetic acid in acetoni...

Embodiment 3

[0108] Example 3 Artemisia argyi active ingredient preparation

[0109] Get the effective components of Folium Artemisiae Argyi in Example 1, 1, 2, 3, 4, or 5, 0.5g and 10.5g of polyethylene glycol-6000, mix evenly, heat and melt, and move to the drip irrigation after the chemical material, and the medicine liquid drops Put it in liquid paraffin at 6-8°C, remove oil, and make 400 dropping pills.

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PUM

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Abstract

The invention relates to an effective component of artemisia leaves as well as a preparation method and an application thereof. The preparation process of the effective component of the artemisia leaves comprises the following steps: (1) a mixture of ethyl acetate and ethanol is used as a solvent to extract the artemisia leaves; (2) dregs of a decoction are extracted with the ethanol so as to obtain an extracting solution; (3) the extracting solution is processed by chromatography through a chromatographic column to obtain an eluent; (4) the eluent is eluted by prepared liquid chromatogram gradient and is collected for 28.0-32.0 minutes, 36.0-40.0 minutes, 48.0-52.0 minutes, 52.0-56.0 minutes and 52.0-60.0 minutes to obtain the effective component by taking water and acetonitrile as flow phases.

Description

technical field [0001] The invention relates to a traditional Chinese medicine extract for treating tumor diseases, in particular to an effective component extracted from leaves of mugwort, a preparation and a preparation method and use thereof. Background technique [0002] Tumor is a common and frequently-occurring disease, among which malignant tumor is the most serious disease that endangers human health. At present, the treatment of malignant tumors in the industry is mainly based on surgery, radiotherapy, and chemotherapy. However, many chemical anticancer drugs often involve normal cells when they act on target cells, causing serious side effects. The genotoxicity of botanical medicine is not obvious, and Chinese herbal medicine has unique advantages and broad application prospects in anti-cancer and anti-mutation, and Chinese medicine also plays a role that cannot be ignored in the adjuvant treatment of tumors. Paclitaxel is a typical natural compound obtained from ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/282A61P35/00A61K127/00
Inventor 贺庆刘雳程翼宇水文波窦静葛志伟
Owner TIANJIN TASLY PHARMA CO LTD
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