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Purification method of psoralen and isopsoralen

A technology of isopsoralen and psoralen, which is applied in the field of extraction and purification of traditional Chinese medicine, and can solve the problems of optic nerve damage and inability to operate in practice.

Inactive Publication Date: 2009-09-16
SHANGHAI UNIV OF T C M
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Methanol is a toxic solvent that can cause damage to the optic nerve. Methanol is used several times in the above-mentioned purification process, which cannot be practically operated in large-scale industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Take the above-mentioned medicinal solution with a relative density of 0.80-0.98 and dilute it with distilled water (0.01g of crude drug per milliliter of medicinal solution, 20°C), and pass it through a commercially available HPD600 macroporous adsorption resin, and the diameter-to-column ratio after loading the resin is 1:1.1 , pre-adsorption for 3 hours, the flow rate is 6ml / min. Use 20%-30% ethanol 20-30 times the mass of the resin to elute the impurities first. Then use 50%-60% ethanol 6-8 times the mass of the resin to elute the impurities. Collect 50%-60% of the eluate. After recovering the ethanol, concentrate and dry in vacuum to obtain the enriched product of psoralen and isopsoralen. Through the high-performance liquid phase test, the content of psoralen and isopsoralen is 56.5% through the external standard one-point method test.

Embodiment 2

[0021] Take the above-mentioned medicinal solution with a relative density of 0.80-0.98 and dilute it with distilled water (the amount of crude drug per milliliter of the medicinal solution is 0.01g, 20°C), and pass it through the commercially available AB-8 macroporous adsorption resin, and the diameter-to-column ratio after loading the resin is 1 : 2, pre-adsorption for 3 hours, the flow rate is 6ml / min. Use 30% ethanol 30-40 times the resin mass to elute impurities first. Impurities were then eluted with 60% ethanol 7 times the mass of the resin. 60% of the eluate was collected. After recovering the ethanol, concentrate and dry in vacuum to obtain the enriched product of psoralen and isopsoralen. Through the high-performance liquid phase test, the content of psoralen and isopsoralen is 57.8% through the external standard one-point method test.

Embodiment 3

[0023] Take the above-mentioned medicinal solution with a relative density of 0.80-0.98 and dilute it with distilled water (the amount of crude drug per milliliter of medicinal solution is 0.01g, 20°C), and pass it through the commercially available D-101 macroporous adsorption resin, and the diameter-to-column ratio after loading the resin is 1. : 1.5, pre-adsorption for 3 hours, the flow rate is 6ml / min. Use 40% ethanol 30 times the mass of the resin to elute the impurities first. Impurities were then eluted with 60% ethanol 8 times the mass of the resin. 60% of the eluate was collected. After recovering the ethanol, concentrate and dry in vacuum to obtain the enriched product of psoralen and isopsoralen. Through the high-performance liquid phase test, the content of psoralen and isopsoralen is 52.1% through the external standard one-point method test.

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Abstract

The invention relates to the technical field of traditional Chinese medicine extraction and purification, in particular to a purification method of the effective components in psoralea, i.e. psoralen and isopsoralen. The method firstly uses low-concentration ethanol for eluting impurities after the psoralea extract is absorbed by macroporous absorptive resin, later uses high-concentration ethanol for elution, and then collects high-concentration ethanol eluate, recycles ethanol, dries the residue in vacuum and implementing grinding, and finally obtains the psoralen and isopsoralen rich sediments. By using the purification method of the invention, the total purity of the obtained psoralen and isopsoralen reaches more than 50 percent; and the purification method is applicable to the industrial mass production.

Description

technical field [0001] The invention relates to the technical field of extracting and purifying traditional Chinese medicines, in particular to a method for purifying psoralen and isopsoralen as effective components in psoralen. Background technique [0002] Psoraleae is a commonly used traditional Chinese medicine, which is recorded in "Compendium of Materia Medica" and "Compendium of Materia Medica". Psoralen is the fruit of Psoralea corylifolia L., a leguminous plant, with pungent nature and flavor, bitter, warm, and returns to the kidney and spleen channels. It has the effects of warming the kidney, helping yang, inducing qi, and stopping diarrhea. Traditional Chinese medicine is often used to treat kidney deficiency, cold diarrhea, enuresis, slippery sperm, frequent urination, impotence, cold pain in the waist and knees, deficiency and cold, asthma and cough, external treatment for skin diseases, vitiligo, alopecia, etc. "The Pharmacopoeia of the People's Republic of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D493/04A61K36/487B01D15/30
Inventor 张宁韩杨王拥军江雪芹刘新华
Owner SHANGHAI UNIV OF T C M
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