Smilax saponin, flavonoid extract and preparation method thereof

A technology of Smilax saponins and flavonoids, which is applied in the field of preparation of traditional Chinese medicine extracts, can solve the problems of being unable to adapt to the requirements of the industrialization of traditional Chinese medicine, unable to produce in large quantities, and unsatisfactory effects, etc., to achieve improved operability, Easy to filter, cost-saving effect

Active Publication Date: 2012-02-22
湖南湘泉药业股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In terms of Smilax smilax extract, the paper "Technology Research on Extracting Total Flavonoids from Smilax Smilax" in "Plant Research" 2006 Volume 26 No. 3 discloses a method for extracting total flavonoids from Smilax Smilax, but the research level of this paper is It is relatively shallow, and only the influence of factors such as temperature and ethanol concentration on the extraction rate has been discussed, and the yield is not high; the patent ("Smilax smilax total glycosides extract and its preparation method and use" application number: 200510095149.3) discloses An extract of total glycosides of Smilax cocos and its preparation method and application, but this method only studies the extract of total glycosides, the yield is low, and it cannot meet the requirements of the industrialization of traditional Chinese medicine
"Study on the Enrichment and Purification of Total Flavonoids of Smilax Smilaxe by Macroporous Adsorption Resin" in "Pharmacy Today" 2008 Volume 18, Issue 3 and "Separation and Purification of Total Flavonoids from Smilax Smilax Smilax" in "Chinese Journal of Traditional Chinese Medicine" 2008 Volume 33 10th Issue 》Respectively disclosed a method for separating and purifying total flavonoids from Smilax smilax alcoholic extract, but these two methods have the problem of high cost and cannot be produced in large quantities
[0005] On the other hand, enzyme preparations have been used in plant extraction and traditional Chinese medicine (such as ginkgo, ginseng, etc.), and have played an important role in the industrialization of traditional Chinese medicine, but most of them use a single enzyme preparation, and the effect is not satisfactory

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] 1. Vacuum-dry the raw medicinal material of Smilax tuckahoe to 5.5% moisture content, dry at 50°C, pulverize to 10 mesh, add 3 times the amount of enzymatic hydrolysis solution, and enzymolyze at 35°C for 9.5 hours. The preparation method of the enzymolysis solution is as follows: dissolving 1.5 grams of plant extract enzyme (19000 units of enzyme activity) in 500 ml of buffer solution with a pH value of 4.2. The plant extract enzyme composition is 30% of cellulase, 40% of pectinase, 10% of protease and 20% of xylanase.

[0015] 2. Use 5 times the weight of Smilax smilax, and the concentration is 60% ethanol to carry out reflux extraction for 3 hours, repeat three times, combine the extracts, concentrate into a paste, degrease with petroleum ether, extract twice with chloroform, combine the extracts, and concentrate and vacuum-dried to obtain flavonoid extracts; then extracted twice with ethyl acetate and combined extracts, concentrated and vacuum-dried to obtain saponi...

Embodiment 2

[0018] 1. Vacuum-dry the raw medicinal material of Smilax smilax to 7% moisture content, dry at a temperature of 55 degrees, pulverize to 18 mesh, add 8 times the amount of enzymatic hydrolysis solution, and perform enzymolysis at a temperature of 45 degrees for 4.5 hours. The preparation method of the enzymolysis solution is as follows: dissolving 8 grams of plant extract enzyme (21000 units of enzyme activity) in 500 ml of buffer solution with a pH value of 5.0. The plant extract enzyme composition is 47% of cellulase, 21% of pectinase, 8% of protease and 24% of xylanase.

[0019] 2. Use 8 times the weight of Smilax smilax, and the concentration is 85% ethanol to carry out reflux extraction for 2.5 hours, repeat three times, combine the extracts, concentrate into a paste, degrease with petroleum ether, extract twice with chloroform, combine the extracts, and concentrate and vacuum-dried to obtain flavonoid extracts; then extracted twice with ethyl acetate and combined extrac...

Embodiment 3

[0022] 1. Vacuum-dry the raw medicinal material of Smilax smilax to 9% moisture content, dry at 65°C, pulverize to 11 mesh, add 10 times the amount of enzymatic hydrolysis solution, and enzymolyze at 48°C for 5 hours. The preparation method of the enzymolysis solution is as follows: 3 grams of plant extract enzyme (30,000 units of enzyme activity) are dissolved in 500 ml of a buffer solution with a pH value of 7. The plant extract enzyme composition is 48% of cellulase, 25% of pectinase, 17% of protease and 10% of xylanase.

[0023] 2. Use 5 times the weight of Smilax smilax, and the concentration is 78% ethanol to carry out reflux extraction under the assistance of ultrasonic waves for 3.6 hours, repeat three times, combine the extracts, concentrate into a paste, degrease with petroleum ether, extract 2 times with chloroform and combine The extract was concentrated and vacuum-dried to obtain a flavonoid extract; then extracted twice with ethyl acetate and the combined extract...

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PUM

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Abstract

The invention discloses a smilax saponin, a flavonoid extract and a preparation method thereof. The method comprises (1) processing the raw material of the smilax, including vacuum drying, crushing and enzymatic hydrolysis of the raw material. (2) The extraction of smilax saponins and flavonoids is refluxed with ethanol under the assistance of ultrasonic waves and then combined and concentrated, degreased with petroleum ether and extracted with chloroform and ethyl acetate respectively, and vacuum-dried to obtain the finished product after recovering the solvent. The flavonoid extract of Poria cocos contains 30-80% of flavonoid compounds; the obtained saponin extract of Smilax cocos contains 20-70% of saponin compounds. The present invention is characterized in that compound plant extract enzyme is used for pretreatment, and it is equipped with ultrasonic assisted extraction technology, so that the utilization of raw materials and medicinal materials is high, the product yield is high, the energy consumption is low, and the operation is simple and convenient. Extracts of flavonoids and saponins are widely used in the fields of raw materials, intermediates and health food, and have broad market prospects.

Description

technical field [0001] The invention relates to a preparation method of a traditional Chinese medicine extract, in particular to a preparation method of extracting saponins and flavonoids from Smilax smilax. Background technique [0002] Smilax smilax is the dried rhizome of Smilax glbra Roxb., a plant of the family Liliaceae, but Smilax glbra Roxb. referred to in the present invention also includes white Smilax, that is, the dried rhizome of Smilax, which is a commonly used Chinese herbal medicine, distributed in the Yangtze River Basin and beyond. South and other places, sweet in taste and flat in nature. Smilax smilax and its extracts have the effects of dehumidification, detoxification, and joint benefit. They are mainly used for damp-heat gonorrhea, carbuncle, scabies, and limb spasms and pain in muscles and bones caused by mercury poisoning. [0003] The main effective components of Smilax smilax and its extracts are saponins and flavonoids. Therefore, the development...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/896A61K125/00A61P29/00A61P19/02A61P17/00
Inventor 胡江宇谢新平谢勇朱晓妮王锡炳
Owner 湖南湘泉药业股份有限公司
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